Anotasi serta analisis gen-gen terkait adhesi pada Lactiplantibacillus plantarum SU-KC1a dan analisis kemampuan adhesinya pada mukus babi = Annotation and Analysis of Adhesion-Related Genes in Lactiplantibacillus plantarum SU-KC1a and Analysis of Its Adhesion to Porcine Mucus

Nitbani, Apdrie Calyca Jacquiline (2022) Anotasi serta analisis gen-gen terkait adhesi pada Lactiplantibacillus plantarum SU-KC1a dan analisis kemampuan adhesinya pada mukus babi = Annotation and Analysis of Adhesion-Related Genes in Lactiplantibacillus plantarum SU-KC1a and Analysis of Its Adhesion to Porcine Mucus. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Kemampuan bakteri probiotik bertahan hidup dalam kondisi saluran pencernaan yang dinamis sangat bergantung pada kemampuannya berkoloni, yang mana tergantung pada kemampuan adhesi bakteri ke dinding saluran pencernaan. Untuk melakukan adhesi, bakteri dapat mengekspresikan molekul yang berfungsi membantu pelekatannya ke dinding usus, antara lain MucBP, FnBP, dan CnBP. Penelitian ini dibagi menjadi dua komponen: 1) menganalisis kemampuan adhesi Lactiplantibacillus plantarum SU-KC1a pada mukus babi menggunakan metode adherence assay, dan 2) menganalisis gen-gen dari genom SU-KC1a yang berperan dalam adhesi. Metode yang digunakan adalah adherence assay menggunakan microplates yang telah diimobilisasi masing-masing dengan BSA dan mukus babi untuk mengetahui persentase adhesi SU-KC1a. Selanjutnya, dilakukan perakitan, pemolesan, dan anotasi data WGS SU-KC1a serta analisis perbandingannya dengan strain SK151 untuk memperoleh informasi mengenai keberadaan gen yang dapat mengekspresikan protein terkait adhesi. Hasil adherence assay menunjukkan bahwa persentase adhesi SU-KC1a pada mukus babi setelah inkubasi 1, 2, dan 3 jam, masing-masing sebanyak 81,19%, 85,14, dan 88,12%. Sedangkan pelekatan SU-KC1a pada BSA setelah inkubasi 1, 2, dan 3 jam masing-masing sebanyak 68,60, 70,24%, dan 67,93%. Hasil tersebut menunjukkan bahwa terjadi peningkatan persentase adhesi pada mukus yang proporsional dengan waktu inkubasi adhesi. Hasil analisis anotasi genom SU-KC1a menunjukkan bahwa terdapat sejumlah CDS yang mengkodekan protein untuk permukaan sel: hydrolase (5 CDS), protein yang berperan dalam proses daur ulang produk peptidoglikan turnover (1 CDS), fibronectin/fibrinogen binding protein (4 CDS), dan surface protein yang belum diketahui fungsinya (13 CDS). Analisis lebih lanjut perbandingan genom SU-KC1a dan SK151 menunjukkan adanya 3 CDS yang memiliki kemiripan bervariasi dengan gen-gen MucBP domain-containing protein pada SK151. / The survivability of probiotic bacteria in dynamic condition of digestive tract is highly dependent on their ability to colonize, which depends on its adhesion to digestive tract wall. To perform adhesion, bacteria can express molecules to help them adhere to the intestinal wall, including MucBP, FnBP, and CnBP. This study was divided into two parts: 1) analyzing the adhesion of Lactiplantibacillus plantarum SU-KC1a to intestinal porcine mucus using the adherence assay method, and 2) analyzing the genes from the SU-KC1a genome that play a role in adhesion. The method used is adherence assay using microplates that have been immobilized with BSA and porcine mucus, respectively, to determine the percentage of SU-KC1a adhesion. Furthermore, the WGS data of SU-KC1a was assembled, polished, annotated, and then analyzed in comparison with the strain SK151 to obtain information about the presence of genes that can express adhesion-related proteins. The adherence assay results showed that the percentage of SU-KC1a adhesion to porcine mucus after incubation for 1, 2, and 3 hours was 81,19%, 85,14, and 88,12%, respectively. Meanwhile, the adhesion of SU-KC1a to BSA after incubation for 1, 2, and 3 hours was 68,60, 70,24%, and 67,93%, respectively. These results indicate that there is an increase in the percentage of adhesion to mucus which is proportional to the incubation time of adhesion. The results of the annotation analysis of the SU-KC1a genome showed that there are several CDS that encode proteins for the cell surface, such as: hydrolase (5 CDS), protein that plays a role in the recycling process of peptidoglycan turnover product (1 CDS), fibronectin/fibrinogen binding protein (4 CDS), and surface proteins with unknown function (13 CDS). Further comparison analysis of the SU-KC1a and SK151 genomes revealed the presence of 3 CDS with varying similarities to the MucBP domain-containing protein genes in SK151.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Nitbani, Apdrie Calyca JacquilineNIM01113180024apdrienitbani@gmail.com
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorTan, Tjie JanNIDN0318104902tjiejan.tan@gmail.com
Thesis advisorVictor, HansNIDN0309049301hans.victor@uph.edu
Uncontrolled Keywords: Lactiplantibacillus plantarum; Adherence assay; Mukus babi; MucBP
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Users 5016 not found.
Date Deposited: 24 Feb 2022 02:23
Last Modified: 24 Feb 2022 03:29
URI: http://repository.uph.edu/id/eprint/46699

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