Aktivitas fibrinolitik dari enzim papain komersial = Fibrinolytic activity of commercial papain enzyme

Vidian, Valerie (2022) Aktivitas fibrinolitik dari enzim papain komersial = Fibrinolytic activity of commercial papain enzyme. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Terbentuknya gumpalan darah yang tidak diinginkan tanpa regulasi yang baik merupakan penyebab beberapa penyakit letal, seperti penyakit jantung, emboli paru-paru, dan stroke. Pengobatan saat ini masih memiliki beberapa kelemahan, seperti efek sampingnya yang cukup mematikan atau harga yang terlalu mahal, sehingga penelitian untuk menemukan alternatif dengan efek samping yang ringan dan harga yang terjangkau perlu dilakukan. Carica papaya adalah tanaman berbuah yang banyak ditemukan di daerah tropis, termasuk Indonesia. C. papaya mengandung beberapa enzim, salah satunya adalah enzim papain. Enzim papain ditemukan memiliki potensi aktivitas fibrinolitik. Maka, dilakukan studi lebih lanjut untuk mengetahui aktivitas fibrinolitik enzim papain yang dijual secara komersial. Dalam penelitian ini, pengujian aktivitas fibrinolitik dilakukan dengan uji degradasi gumpalan darah dan euglobulin, fibrin plate method, SDS-PAGE, dan zimografi. Hasil menunjukkan bahwa enzim papain komersial yang digunakan diketahui memiliki kemampuan untuk mendegradasi fibrin secara langsung. / The formation of unwanted blood clot inside the body without good regulation is the major cause of some deadly complications, such as heart attack, pulmonary embolism, and stroke. Current medication for these circumstances has several weaknesses, some enacts lethal side effects or too high of a price, so research in finding alternatives with mild to no side effects with affordable prices are needed. Carica papaya is one of a fruiting plant which can easily be found in tropical areas, including Indonesia. C. papaya contains several enzymes, one of which is the papain enzyme. Papain enzyme has been found to have a fibrinolytic ability. Therefore, further studies were conducted to determine the fibrinolytic activity of the commercially sold papain enzyme. In this study, fibrinolytic activity of the sample was tested using blood clot and euglobulin clot lysis tests, fibrin plate method, SDS-PAGE, and zymography. The results indicate that the commercial papain enzyme used and treated in this study can degrade fibrin directly.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Vidian, ValerieNIM01113180008valerievidian@gmail.com
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPinontoan, ReinhardNIDN0325046502UNSPECIFIED
Thesis advisorKiranadi, BambangNIDN0026125004UNSPECIFIED
Uncontrolled Keywords: Antitrombotik; Carica papaya; Degradasi fibrin; Enzim papain komersial; Fibrinolitik; Antithrombotic; Carica papaya; Commercial papain enzyme; Fibrin degradation; Fibrinolytic
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Users 5001 not found.
Date Deposited: 26 Feb 2022 01:51
Last Modified: 26 Feb 2022 01:51
URI: http://repository.uph.edu/id/eprint/46812

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