Analisa enzim amilase dan protease tahan basa dari Bacillus sp. untuk aplikasi biodeterjen = Analysis of alkaline amylase and protease from Bacillus sp. in biodetergent perspective

Baskoro, Abyatar Hugo (2014) Analisa enzim amilase dan protease tahan basa dari Bacillus sp. untuk aplikasi biodeterjen = Analysis of alkaline amylase and protease from Bacillus sp. in biodetergent perspective. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Eutrophication is a phenomenon in which an explosion of algae growth occurs. This phenomenon is detrimental in aquatic ecosystems because it pollutes the waters and therefore unsuitable for organisms to live. The high phosphate content of detergents causes phosphate waste and this triggers the growth of algae. Moreover, in detergents there are also dangerous chemicals that can pollute the environment such as sodium hypochlorite and hydrogen peroxide. One of many solution to this problem is biodetergent. Biodetergent is a biodegradabel detergent that contains enzymes. The enzyme could enhance the wash performance of the detergent in stain removal so that the phosphate compound in detergents and hazardous materials can be reduced. In this study, the suitability of the enzyme amylase and protease from five UPH’s Bacillus sp. collection for biodetergent applications is investigated. The protease and amylase tested is stable at pH 9-12 and stable at temperatures 25-60oC. Ca2+ increases the activity of amylase significantly. While protease is generally not affected Ca2+, Mg2+, Fe2+, Zn2+, and Hg2+ metal ion. Detergent content of 7 mg/ml significantly inhibited the performance of amylase, while there is no significant decrease in protease activity. Stain removal test results prove that all the amylase of Bacillus sp. can remove strach stains and therefore suitable for biodetergent application. In protease case, only three Bacillus sp. protease that can remove blood stains and therefore suitable for biodetergent application.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Baskoro, Abyatar HugoUNSPECIFIEDUNSPECIFIED
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPinontoan, ReinhardUNSPECIFIEDUNSPECIFIED
Thesis advisorTan, Tjie JanUNSPECIFIEDUNSPECIFIED
Additional Information: SK 113-10 BAS a
Uncontrolled Keywords: amylase ; protease ; alkaline ; Bacillus sp. ; biodetergent
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Ms Monica Putri Damayanti
Date Deposited: 04 Oct 2018 05:39
Last Modified: 16 Aug 2021 07:15
URI: http://repository.uph.edu/id/eprint/506

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