Identifikasi dan karakterisasi bakteri tahan panas penghasil enzim pencernaan dari sistem pencernaan babi = Identification and characterization of digestive enzymes producing heat-tolerant bacterias from pig’s gastrointestinal tract

Rivan, Rivan (2014) Identifikasi dan karakterisasi bakteri tahan panas penghasil enzim pencernaan dari sistem pencernaan babi = Identification and characterization of digestive enzymes producing heat-tolerant bacterias from pig’s gastrointestinal tract. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

As a monogastric omnivore, pig’s diet consists of plants and small insects. However, since pig’s gastrointestinal tract is incapable to digest most of the substrate consumed, a mutualistic symbiosis between microorganisms in the digestive system would most likely to occur. This will result on a very large and diverse species of gut microbiota most of which is able to produce digestive enzymes. The study of these gut microbiota from pig’s intestine has not been widely explored in Indonesia. Thus, this study aims to identify heat-tolerant bacterias with beneficial properties for potential feed additives. A total of 145 heat-tolerant bacterias were isolated from six different parts of pig intestine. Preliminary protease and amylase screening narrowed down the number of isolates to 4 groups representatives. Based on morphological and biochemical assay, the 4 isolates were predicted to be from the genus of Bacillus. Molecular identification of the 16S rRNA gene of the 4 isolates resulted in an inconclusive result due to high similarity between Bacillus species. The 4 chosen isolates were predicted to be B. amyloliquefaciens D9, Bacillus amyloliquefaciens I15, B. pumilus J2, and Bacillus methylotrophicus R12

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