Analisis pengaruh mikroenkapsulasi dengan matriks alginat-kitosan dan pektin-kitosan terhadap viabilitas Bifidobacterium breve BS2-PB3 setelah freeze drying = Analysis of the effect of microencapsulation with alginate-chitosan and pectin-chitosan on the viability of Bifidobacterium breve BS2-PB3 after freeze drying

Celine, Patricia (2023) Analisis pengaruh mikroenkapsulasi dengan matriks alginat-kitosan dan pektin-kitosan terhadap viabilitas Bifidobacterium breve BS2-PB3 setelah freeze drying = Analysis of the effect of microencapsulation with alginate-chitosan and pectin-chitosan on the viability of Bifidobacterium breve BS2-PB3 after freeze drying. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Bifidobacterium breve umum digunakan sebagai probiotik. Probiotik dapat mengalami penurunan viabilitas ketika terpapar kondisi pencernaan seperti pH rendah, enzim pencernaan, dan melewati proses pengolahan pada bidang industri serta penyimpanan. Mikroenkapsulasi sering digunakan untuk meningkatkan viabilitas probiotik. Penelitian ini bertujuan untuk menganalisis pengaruh mikroenkapsulasi dengan matriks alginat-kitosan dan pektin-kitosan terhadap viabilitas Bifidobacterium breve BS2-PB3 setelah freeze drying; menganalisis pengaruh pemberian mikrokapsul BS2-PB3 pada perubahan pH, berat, dan jumlah dropping feses mencit BALB/c. Prosedur penelitian ini terdiri dari preparasi kultur BS2-PB3; mikroenkapsulasi BS2-PB3; uji viabilitas BS2-PB3 sebelum dan sesudah mikroenkapsulasi dan freeze drying; uji shelf life; uji toleransi terhadap simulated gastric juice (SGJ) dan garam empedu; analisis pengaruh pemberian mikrokapsul BS2-PB3 pada perubahan pH, berat, dan jumlah dropping feses mencit BALB/c Hasil yang diperoleh adalah mikrokapsul BS2-PB3 dengan matriks alginat-kitosan dan pektin-kitosan berhasil dibuat menggunakan metode ekstrusi. Mikroenkapsulasi dapat mempertahankan viabilitas BS2-PB3 hingga lebih dari 96% setelah freeze drying. Kedua matriks dapat melindungi BS2-PB3 selama 56 hari masa penyimpanan, dengan persentase survivabilitas di atas 96% dan 83% untuk penyimpanan pada suhu 4°C dan suhu ruang. Selain itu, kedua matriks juga dapat menjaga viabilitas BS2-PB3 setelah terpapar SGJ (55%) dan garam empedu (66%). Pemberian mikrokapsul BS2-PB3 selama 21 hari memberikan efek signifikan terhadap penurunan pH dan peningkatan berat serta jumlah dropping feses mencit. Penelitian ini menunjukkan bahwa teknik mikroenkapsulasi menggunakan matriks alginat-kitosan dan pektin-kitosan dilanjutkan dengan pengeringan terbukti dapat meningkatkan viabilitas serta stabilitas probiotik. / Bifidobacterium breve is a bacterial species commonly used as probiotics. However, probiotics can experience a decrease in viability when exposed to digestive conditions such as low pH, digestive enzymes, and industrial processing and storage. Microencapsulation is a technique that can be used to increase the viability of probiotics. This study aims to analyse the effect of microencapsulation with alginate-chitosan and pectin-chitosan matrices on the viability of BS2-PB3 after freeze drying and analyse the effect of BS2-PB3 microcapsules on changes in pH, weight, and the amount of feces in BALB/c mice. The research procedure consisted of: BS2-PB3 culture preparation; BS2-PB3 microencapsulation; bacterial viability test before and after microencapsulation and freeze drying; shelf life test; tolerance test to simulated gastric juice and bile salts; and analysis of the effect of BS2-PB3 microcapsule administration on changes in pH, weight, and the amount of feces in BALB/c mice. The results obtained were BS2-PB3 microcapsules with alginate-chitosan and pectin-chitosan matrices successfully made using the extrusion method. Microencapsulation can maintain BS2-PB3 viability up to more than 96% after freeze drying. Both matrices can protect BS2-PB3 for 56 days of storage, with a survival rate of more than 96% and 83% for storage at 4°C and room temperature, respectively. In addition, the two matrices can also maintain BS2-PB3 viability after exposure to SGJ (55%) and bile salts (66%). After receiving BS2-PB3 microcapsules for 21 days, the fecal pH was significantly lower than the control group, but the weight and volume were both significantly increased. This study shows that microencapsulation technique using alginate-chitosan and pectin-chitosan matrices followed by drying is proven to increase the viability and stability of probiotics.

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