Analisis kandidat probiotik Bifidobacterium breve BS2-PB5 pada berbagai kondisi garam empedu, pH, dan suhu = Analysis of probiotic candidate Bifidobacterium breve BS2-PB5 under various bile salt, pH, and temperature conditions

Huang, Theresa Florensia (2023) Analisis kandidat probiotik Bifidobacterium breve BS2-PB5 pada berbagai kondisi garam empedu, pH, dan suhu = Analysis of probiotic candidate Bifidobacterium breve BS2-PB5 under various bile salt, pH, and temperature conditions. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Probiotik adalah mikroorganisme hidup yang dapat memberikan manfaat kesehatan bagi inang apabila diberikan dalam jumlah yang cukup. Agar dapat memberikan efek kesehatan bagi inang, probiotik harus bertahan hidup dalam kondisi ekstrim pada saluran pencernaan dan pemrosesan industri. Bifidobacterium breve merupakan probiotik yang banyak digunakan untuk kesehatan, seperti pada produk yoghurt dan suplemen probiotik. Pada penelitian ini dilakukan pengamatan morfologi dan pertumbuhan B. breve BS2-PB5 serta evaluasi beberapa sifat-sifat probiotik dasar, seperti ketahanan terhadap berbagai konsentrasi garam empedu, pH, dan suhu. Tahapan penelitian ini meliputi: (1) Pengamatan pertumbuhan dengan kondisi anaerob selama 72 jam, (2) Pengujian ketahanan terhadap garam empedu (0%, 0,3%, dan 0,5%), (3) Pengujian terhadap berbagai pH (2, 5, 7,5), (4) Pengujian ketahanan terhadap suhu tinggi (42°C, 45°C, 48°C). Hasil yang diperoleh menunjukkan bahwa BS2-PB5 dapat bertahan terhadap paparan garam empedu konsentrasi 0,5%. Penelitian terhadap pH rendah menunjukkan bahwa BS2-PB5 tidak dapat hidup pada pH 2, tetapi dapat bertahan hidup pada pH 5 dan pH 7,5. Pada penelitian pertumbuhan BS2-PB5 pada suhu tinggi menunjukkan sel dapat hidup dan masih bertumbuh dengan baik pada suhu 42°C, tetapi tidak dapat tumbuh dengan baik pada suhu 45°C dan 48°C. Hasil yang diperoleh mengindikasikan bahwa BS2-PB5 memenuhi kriteria dasar probiotik. / Probiotics are live microorganisms that can deliver health benefits to the host when they are administered in sufficient amounts. In order to exert health effects on the host, probiotics must be able to survive the extreme conditions of the gastrointestinal tract and through the industrial processing. Bifidobacterium breve is one of the probiotics that is widely used for health, such as in yogurt products and probiotic supplements. This study observed the morphology and growth of B. breve BS2-PB5 and evaluates some basic probiotic properties, such as resistance to various bile salt concentrations, pH, and temperature. The steps of this research consisted of: (1) Observation of growth under anaerobic conditions for 72 hours, (2) Testing for resistance to bile salts (0%, 0.3%, and 0.5%), (3) Testing for various pH (2, 5, 7,5), (4) Testing for resistance to high temperatures (42°C, 45°C, 48°C). The results obtained showed that BS2-PB5 could withstand exposure to 0.5% concentration of bile salts. Tests at low pH showed that BS2-PB5 could not survive at pH 2, but could survive at pH 5 and pH 7.5. Research on the growth of BS2-PB5 at high temperatures showed that the cells could survive and continue to grow well at 42°C, but could not grow well at 45°C and 48°C. The results obtained indicates that BS2-PB5 meets the basic criteria of probiotics.

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