Identifikasi dan evaluasi enzim fitase dari bakteri Bacillus sp. koleksi Universitas Pelita Harapan = Identification and Evaluation of phytase produced by bacteria Bacillus sp. from Universitas Pelita Harapan culture collection

Tan, Steven Ryan Susanto (2015) Identifikasi dan evaluasi enzim fitase dari bakteri Bacillus sp. koleksi Universitas Pelita Harapan = Identification and Evaluation of phytase produced by bacteria Bacillus sp. from Universitas Pelita Harapan culture collection. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Phytate in animal feedstuff are not subjected to be digested by monogastrics, so the incorporation of phytase is needed to maximize feeding efficiency. Although a vast diversity of phytase-producing microorganisms resides in Indonesia, the demand for phytase fullfilment for livestock is still much dependent on other countries. The research aim is to evaluate the ability of Bacillus sp. from Universitas Pelita Harapan culture collection to produce phytase, using qualitative and quantitative tests as well as further characterization of the phytase produced, by observing the effect of temperature and pH against the enzyme activity, and identifying the molecular weight of the enzyme. From the results of this research, it is known that B. amyloliquefaciens, B. methylotropicus and B. subtilis are able to produce phytase. Further characterization is performed in the phytase produced by B. amyloliquefaciens N1. B amyloliquefaciens N1 phytase activity is relatively stable at temperatures 30 to 60 °C and at pH 4 to 8. Protein separation of crude enzyme extract using SDS-PAGE shows protein expression that is induced by phytate in synthetic minimal medium, and can be predicted as phytase with molecular weight 41 kDa or 44,5 kDa.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Tan, Steven Ryan SusantoUNSPECIFIEDUNSPECIFIED
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPinontoan, ReinhardUNSPECIFIEDUNSPECIFIED
Thesis advisorJap, LucyUNSPECIFIEDUNSPECIFIED
Uncontrolled Keywords: Identification ; evaluation ; phytase ; Bacillus sp.
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Ms Monica Putri Damayanti
Date Deposited: 04 Oct 2018 05:42
Last Modified: 21 Feb 2019 08:28
URI: http://repository.uph.edu/id/eprint/582

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