Uji antibakteri kombinasi ekstrak daun teh hijau (Camellia sinensis Linn. Kuntze) dan ekstrak bawang putih (Allium sativum Linn.) terhadap methicillin-resistant Staphylococcus aureus dan Staphylococcus aureus

Wijaya, Wilson (2024) Uji antibakteri kombinasi ekstrak daun teh hijau (Camellia sinensis Linn. Kuntze) dan ekstrak bawang putih (Allium sativum Linn.) terhadap methicillin-resistant Staphylococcus aureus dan Staphylococcus aureus. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Resistensi merupakan masalah global yang terjadi di dunia karena dapat terjadi pada semua mikroba, salah satunya bakteri Staphylococcus aureus yang menjadi Methicillin-resistant Staphylococcus aureus dengan prevalensi yang tinggi secara global. Hal ini disebabkan oleh penggunaan antibiotik beta laktam yang tidak disiplin. Untuk mengatasi permasalahan ini dapat digunakan pengobatan herbal yang mengandung sifat antibakteri yang efektif. Camellia sinensis Linn. memiliki metabolit sekunder senyawa epigalokatekin galat (EGCG), dan Allium sativum Linn. memiliki metabolit sekunder senyawa allicin, yang keduanya telah diteliti sebelumnya terdapat aktivitas antibakteri dengan spektrum yang luas. Ekstrak etanol 75% Camellia sinensis Linn. melalui refluks dan ekstrak etanol 96% Allium sativum Linn. melalui maserasi. Pengujian nilai Konsentrasi Hambat Minimum (KHM) dilakukan dengan metode mikrodilusi, lalu nilai Konsentrasi Bunuh Minimum (KBM) dengan media agar. Kedua ekstrak dikombinasikan dengan rasio 1:1, 1:2, dan 2:1 dengan harapan diperoleh kombinasi ekstrak yang lebih efektif. Dari hasil penelitian didapat nilai KHM90 hanya dari ekstrak kombinasi 1:2 dengan konsentrasi 3.333,333 ppm terhadap bakteri S.aureus dan MRSA. Namun nilai KBM tidak dapat diperoleh dikarenakan nilai KHM90 yang telah ada pada konsentrasi tertinggi./Resistance is a global problem that occurs in the world because it can occur in all microbes, one of which is the bacteria Staphylococcus aureus which becomes Methicillin-resistant Staphylococcus aureus with a high prevalence globally. This is caused by the undisciplined use of beta lactam antibiotics. To overcome this problem, herbal remedies that contain effective antibacterial properties can be used. Camellia sinensis Linn. has a secondary metabolite compound epigalocatechin gallate (EGCG), and Allium sativum Linn. has a secondary metabolite compound allicin, both of which have been previously studied to have broad-spectrum antibacterial activity. 75% ethanol extract of Camellia sinensis Linn. through reflux and 96% ethanol extract of Allium sativum Linn. through maceration. The test of Minimum Inhibitory Concentration (MIC) value was done by microdilution method, then Minimum Bactericidal Concentration (MBC) value with agar media. Both extracts were combined with a ratio of 1:1, 1:2, and 2:1 in the hope of obtaining a more effective combination of extracts. From the results of the study, the MIC90 value was obtained only from the 1:2 combination extract with a concentration of 3,333.333 ppm against S.aureus and MRSA bacteria. However, the MBC value cannot be obtained because the MIC90 value already exists at the highest concentration.

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