Analisis fisiologis dan molekular sifat resistansi Pseudomonas aeruginosa PaD2 terhadap tembaga dan antibiotik = Physiological and molecular analysis of resistance properties of Pseudomonas aeruginosa pad2 to copper and antibiotiks

Umbu Datta, Dian Angelica Tryoanita (2024) Analisis fisiologis dan molekular sifat resistansi Pseudomonas aeruginosa PaD2 terhadap tembaga dan antibiotik = Physiological and molecular analysis of resistance properties of Pseudomonas aeruginosa pad2 to copper and antibiotiks. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Pencemaran lingkungan oleh tembaga dan antibiotik memerlukan solusi yang efisien dan ramah lingkungan. Bioremediasi menggunakan bakteri multiresisten, seperti Pseudomonas aeruginosa PaD2, adalah pendekatan yang menjanjikan. Penelitian ini bertujuan untuk memahami mekanisme co-resistance pada Pseudomonas aeruginosa PaD2 dengan mengidentifikasi gen resistansi dan mengevaluasi efek tembaga (II) sulfat terhadap resistansi antibiotik. Tujuan khususnya termasuk menentukan profil resistansi antibiotik, Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC), serta mengidentifikasi gen resistansi melalui bioinformatika. Penelitian ini penting untuk memastikan potensi penggunaan Pseudomonas aeruginosa PaD2 sebagai agen bioremediasi. Hasil penelitian menunjukkan bahwa Pseudomonas aeruginosa PaD2 resistan terhadap sebagian besar antibiotik yang diuji kecuali gentamicin, sulfonamide, ofloxacin, ciprofloxacin (MIC >0,5 mg/L; MBC >4 mg/L), dan tetracycline (MIC >64 mg/L; MBC >64 mg/L). Diidentifikasi mekanisme resistansi meliputi pompa efflux, inaktivasi antibiotik, dan alterasi target antibiotik. Tidak ada gen resistansi tembaga yang terdeteksi. Ditemukan beberapa insertion sequence yang dapat berperan dalam mobilisasi gen resistansi. Penelitian selanjutnya sebaiknya fokus pada perbandingan media untuk resistansi bakteri dan memahami mekanisme resistansi tembaga. / Environmental pollution by copper and antibiotics requires efficient and environmentally friendly solutions. Bioremediation using multiresistant bacteria, such as Pseudomonas aeruginosa PaD2, is a promising approach. This study aimed to understand the co-resistance mechanism in Pseudomonas aeruginosa PaD2 by identifying resistance genes and evaluating the effect of copper(II) sulphate on antibiotic resistance. Specific objectives include determining antibiotic resistance profiles, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), as well as identifying resistance genes through bioinformatics. This research is important to ensure the potential use of Pseudomonas aeruginosa PaD2 as a bioremediation agent. The results showed that Pseudomonas aeruginosa PaD2 was resistant to most of the antibiotics tested except gentamicin, sulfonamide, ofloxacin, ciprofloxacin (MIC >0.5 mg/L; MBC >4 mg/L), and tetracycline (MIC >64 mg/L; MBC >64 mg/L). Resistance mechanisms including efflux pump, antibiotic inactivation, and antibiotic target alteration were identified. No copper resistance gene was detected. Several insertion sequences were found that could play a role in the mobilisation of resistance genes. Future research should focus on comparing media for bacterial resistance and understanding the mechanism of copper resistance.

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