Potensi isolat bacillus sp. sebagai agen fibrinolitik dan antitrombotik = Potential of bacillus sp. Isolates as fibrinolytic and antithrombotic agents

Elvina, Elvina (2019) Potensi isolat bacillus sp. sebagai agen fibrinolitik dan antitrombotik = Potential of bacillus sp. Isolates as fibrinolytic and antithrombotic agents. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Penyakit kardiovaskular merupakan salah satu penyebab kematian tertinggi di dunia. Namun agen terapeutik yang umum digunakan hingga saat ini masih memiliki beberapa kelemahan, sehingga diperlukan studi terhadap agen alternatif yang lebih efisien. Bacillus sp. diketahui sebagai bakteri yang tidak hanya memiliki aktivitas fibrinolitik melainkan juga antitrombotik. Oleh karena itu, studi lebih lanjut dilakukan untuk mengevaluasi potensi berbagai isolat Bacillus sp. koleksi Program Studi Biologi Universitas Pelita Harapan sebagai agen fibrinolitik dan antitrombotik. Pada penelitian ini dilakukan uji degradasi gumpalan darah, uji aktivitas fibrinolitik, dan uji aktivitas antitrombotik terhadap Bacillus amyloliquefaciens N1, Bacillus amyloliquefaciens SS3.4, Bacillus subtilis IFP1.1, dan Bacillus subtilis natto. Keempat isolat yang digunakan diketahui mampu mendegradasi gumpalan darah akibat aktivitas fibrinolitik dari sampel. Keempat isolat dapat mendegradasi fibrin secara langsung dan kedua isolat yaitu Bacillus amyloliquefaciens N1 dan Bacillus subtilis natto juga berperan sebagai plasminogen aktivator. Selain memiliki aktivitas fibrinolitik, keempat isolat diketahui memiliki aktivitas antitrombotik yaitu mencegah terbentuknya gumpalan darah yang diduga akibat adanya aktivitas hidrolisis fibrinogen dari sampel. Baik aktivitas fibrinolitik maupun antitrombotik, aktivitas tertinggi dimiliki oleh Bacillus subtilis IFP1.1 diikuti dengan Bacillus subtilis natto, Bacillus amyloliquefaciens N1, dan Bacillus amyloliquefaciens SS3.4. Berdasarkan penelitian ini, Bacillus subtilis IFP1.1 memiliki potensi tertinggi sebagai agen fibrinolitik dan antitrombotik. / Cardiovascular disease is one of the highest causes of mortality in the world. The application of various therapeutic agents has been done, but they still have several limitations, thus studies of alternative agents are needed. Bacillus sp. has been known as a bacteria that does not only have fibrinolytic but also antithrombotic activity. Therefore, this study was conducted on the potential of Bacillus sp. isolates from the Biology Department, Pelita Harapan University as fibrinolytic and antithrombotic agents. In this study, blood clot lysis, fibrinolytic, and antithrombotic activities were tested on Bacillus amyloliquefaciens N1, Bacillus amyloliquefaciens SS3.4, Bacillus subtilis IFP1.1, and Bacillus subtilis natto. The four isolates are able to degrade blood clots and fibrin directly while two of four isolates (Bacillus amyloliquefaciens N1 and Bacillus subtilis natto) also act as plasminogen activators. Besides having fibrinolytic activity, the four isolates also have antithrombotic activity which can prevent the formation of blood clots due to their fibrinogen hydrolysis activity. For both fibrinolytic and antithrombotic activities, Bacillus subtilis IFP1.1 has the highest activity followed by Bacillus subtilis natto, Bacillus amyloliquefaciens N1, and Bacillus amyloliquefaciens SS3.4. Based on this study, Bacillus subtilis IFP1.1 has the highest potential as fibrinolytic and antithrombotic agents.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Elvina, ElvinaNIM00000011583elvinaavi@yahoo.co.id
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPinontoan, ReinhardNIDN0325046502UNSPECIFIED
Thesis advisorSanjaya, AstiaNIDN0325068902UNSPECIFIED
Additional Information: SK 113-15 ELV p
Uncontrolled Keywords: antitrombotik; Bacillus sp.; degradasi fibrin; fibrinolitik; hidrolisis fibrinogen; plasminogen aktivator; antithrombotic; fibrin degradation; fibrinolytic; hydrolysis fibrinogen; plasminogen activator
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Mr Samuel Noya
Date Deposited: 16 Mar 2020 07:54
Last Modified: 20 Aug 2021 06:35
URI: http://repository.uph.edu/id/eprint/8391

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