Optimasi produksi n-asetilglukosamin kasar dari kitin kulit udang windu menggunakan enzim kitinase intraseluler kasar = Optimization of crude n-acetylglucosamine production from chitin tiger shrimp shell using intracellular crude enzyme chitinase from providencia stuartii

Sari, Desy Puspa (2018) Optimasi produksi n-asetilglukosamin kasar dari kitin kulit udang windu menggunakan enzim kitinase intraseluler kasar = Optimization of crude n-acetylglucosamine production from chitin tiger shrimp shell using intracellular crude enzyme chitinase from providencia stuartii. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Hidrolisis kitin kulit udang secara enzimatik menggunakan bakteri Providencia stuartii dapat menghasilkan N-asetilglukosamin kasar. Tujuan dari penelitian ini adalah untuk menentukan pH, suhu, dan konsentrasi substrat kitin dengan waktu fermentasi optimum untuk produksi N-asetilglukosamin kasar. Aktivitas enzim intraseluler kasar bakteri Providencia stuartii dianalisis menggunakan metode 3,5 Dinitrosalicylic acid (DNS). PH 3, 4, 5, 6, 7, 8, 9 dan suhu 30, 40, 50, 60, 70, 80 oC digunakan untuk menentukan aktivitas enzim optimum pada Penelitian Tahap I. Hasil penelitian menunjukan bahwa pH dan suhu optimum aktivitas enzim berada pada pH 5 dengan aktivitas enzim sebesar 6,02±0,16 U/ml dan suhu 40 oC dengan aktivitas enzim sebesar 6,03±0,24 U/ml. pH dan suhu optimum kemudian digunakan untuk penelitian lebih lanjut dalam Penelitian Tahap II dengan variasi konsentrasi substrat kitin (0,5; 1; 1,5 dan 2%) dan waktu fermentasi (2, 4, 6, dan 24 jam). Hasil penelitian fermentasi enzimatik mengahasilkan konsentrasi substrat optimum pada 1% dan waktu fermentasi selama 6 jam dengan kadar N-asetilglukosamin kasar tertinggi sebesar 1680,00±58,49 U/ml. / Enzymatic hydrolysis of chitin shrimp using Providencia stuartii bacteria to produce curde N-acetylglucosamine. The purpose of this study was to determine the pH, temperature, and substrate concentration with the best fermentation time for crude N-acetylglucosamine production. Activity of intracellular chitinase crude enzyme was analyzed using 3,5 Dinitrosalicylic acid (DNS) method. The pH of 3, 4, 5, 6, 7, 8, 9 and temperature of 30, 40, 50, 60, 70 dan 80 oC were used for the optimization of enzyme activity in first stage. Results showed that the selected pH and optimum temperature of N-acetylglucosamine production was at pH 5 with enzyme activity of 6,02±0,16 U/ml and temperature of 40oC with enzyme activity of 6,03±0,24 U/ml. The selected pH and the optimum temperature was then used for further research in second stage with variation of chitin concentration substrate (0,5; 1; 1,5 dan 2%) and fermentation time (2, 4, 6, and 24 h). Results showed that the best fermentation was obtained with 1% substrate concentration and fermentation time of 6 ho with high crude N-acetylglucosamine concentration of 1680,00±58,49 U/ml.

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