Genotyping antibiotic resistance patterns in Pediococcus pentosaceus isolates

Boentoro, Josephine (2020) Genotyping antibiotic resistance patterns in Pediococcus pentosaceus isolates. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Probiotics are recognized as an important supplement in the process of rearing livestock, including pigs, as they can improve production, quality, and mitigate illness. In a previous study, two Pediococcus pentosaceus isolates were isolated and studied for their potential as probiotics, including the phenotypic determination of antibiotic resistance profiles. A discrepancy in the phenotypes was detected, despite the isolates having been obtained from the same host: isolate H29 showed lincomycin resistance, while isolate D32 was sensitive to lincomycin. The purpose of this study is to explain the genotypic basis of this discrepancy using whole- genome sequencing of these P. pentosaceus isolates. Genomic DNA was isolated from these and whole-genome sequencing was carried out. Following assembly and annotation, it was found that H29 harbored lnuA, a resistance gene to lincomycin. This lnuA gene had also been detected in the whole-genome sequences of several lactobacilli, and bore 99.7% resemblance to that from Staphylococcus chromogenes. In-silico analysis of the genomic sequence of H29 predicted a plasmid similar to the plasmid pR18 isolated from Lactobacillus plantarum strain PA18. BLAST search of plasmid pR18 against the H29 genome showed 99.97% similarity and coverage. This strongly indicates that P. pentosaceus isolate H29 harbors a plasmid encoding the lnuA gene which confers lincomycin resistance. This plasmid was likely transferred from other lactic acid bacteria although the gene originated from staphylococci. Further study is needed to precisely characterize this plasmid and the genetic basis of other resistance phenotypes observed in these isolates.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Boentoro, JosephineNIM00000024859jboentoro@gmail.com
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorPinontoan, ReinhardNIDN0325046503reinhard.pinontoan@uph.edu
Thesis advisorSanjaya, AstiaNIDN0325068902astia.sanjaya@uph.edu
Additional Information: SK 113-16 BOE g
Uncontrolled Keywords: Antibiotic resistance; genotyping; probiotics
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Users 3794 not found.
Date Deposited: 23 Sep 2020 03:03
Last Modified: 16 Aug 2021 08:08
URI: http://repository.uph.edu/id/eprint/11055

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