This study aims to analyze the impact of Entrepreneurship Education on Entrepreneurial Self-efficacy among undergraduate students in Medan. The subject in this study was undergraduate students who have or are following an entrepreneurship course in their last term of study. This study involved cluster sampling method and resulted to 95 students as a condition of being a respondent. Based on the analysis that has been made the results which are the coefficient of correlation is 0.652 which means that there is positive and strong relationship between Entrepreneurship Education and Entrepreneurial Selfefficacy. The coefficient of determination is 0.425 which means that the Entrepreneurial Self-efficacy can be explained by Entrepreneurship Education in amount of 42.5% and simple linear regression analysis is Y = 7.004+0.927x means that the increasing of Entrepreneurship Education in 1 unit can increase Entrepreneurial Self-Efficacy in 0.927 units. Thus, hypothesis alternative (Ha) is accepted that Entrepreneurship Education has a significant influence on Entrepreneurial Self-efficacy among Undergraduate students in Medan.