Pineapple (Ananas comosus (L.) Merr.) is a fruit that consist of antioxidant useful for our health. The part of pineapple usually consumed is the flesh (about 53%) and the other parts are treated as waste (crown, peel, core, and stem). This study was aimed to observe the antioxidant activity of each part of pineapple. Methanol was used as the solvent for the extraction. The total phenolic, total flavonoid, and antioxidant activity of the extract obtained were analyzed. The result showed that the highest antioxidant activity was found in the peel and crown of the pineapple. The IC50 of peel, crown, stem, flesh, and core of Nanas Subang were 1.0619 mg, 1.1666 mg, 2.6108 mg, 4.2326 mg, and 4.9861 mg, respectively. The antioxidant activity of the peel and crown extract was three to four times higher than the flesh that usually consumed. The antioxidant activity was mostly contributed by phenolic compound especially flavonoid. Besides phenolic content, vitamin C was also found to give contribution to the antioxidant activity especially in the peel, flesh, and core of both pineapples.