Tjoa, Andreas (2012) Effect of extraction method of antioxidant characteristics of sappanwood (caesalpinia sappanl.)Heartwood extract and its incorporation into herbal tea. Bachelor thesis, Universitas Pelita Harapan.
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Abstract
Sappanwood(Caesalpinia sappan L.) is a potential functional food
ingredient, and the production of extract is expected to increase the potency of its
usage. This research was aimed to study the effect of extraction method on
antioxidant characteristics of sappanwood heartwood extracts and their
incorporationinto herbal tea. Sappanwood heartwood was extracted using
maceration and reflux with varying temperature and time regimes. The crude
extracts were subjected toantioxidant activity, total phenolics, total tannin, total
flavonoid, and colorimetric tests.One best candidate in terms of antioxidant
activity from each extraction method was qualitatively assayed for the
phytochemical constituents and incorporatedinto herbal tea.Maceration at 35 oC
for 2 days and reflux at 90 oC for 3 hours resulted in extract with best antioxidant
activity of IC50 = 9.7054 and 7.6963 mg/L, respectively. The extracts were
incorporated at varying concentrations in replacement of conventional bulk
heartwood which served as a control, and were analyzed for their antioxidant
activity. Linear regressions were used to estimate the amount of extractneeded for
each to achieve equal amount of antioxidant activity to the control. Extract
concentrations of 0.3612 g/2.5 L and 0.3420 g/2.5 L were required for macerated
and refluxed extracts respectively to achieve equal antioxidant activity to the
control containing 22.5 g of bulk heartwood in 2.5 L of herbal tea formulation.
The resultant product equivalents and control were then subjected to hedonic test
and colorimetric test.Extract-incorporated formulations showed lighter yellowred color and significantly higher color preference as compared to the control,
but with no significant difference in terms of overall acceptance
Item Type: | Thesis (Bachelor) |
---|---|
Creators: | Creators NIM Email ORCID Tjoa, Andreas NIM03420080093 UNSPECIFIED UNSPECIFIED |
Contributors: | Contribution Contributors NIDN/NIDK Email Thesis advisor Pokatong, Wilbur Donald Raymond NIDN0002106402 UNSPECIFIED Thesis advisor Manuel, Jeremia UNSPECIFIED UNSPECIFIED |
Additional Information: | NIM 03420080093 SK 34-08 TJO e |
Uncontrolled Keywords: | Sappanwood heartwood, Caesalpinia sappan L., extract, maceration, reflux, antioxidant, herbal tea |
Subjects: | T Technology > TP Chemical technology > TP368-456 Food processing and manufacture |
Depositing User: | Users 18 not found. |
Date Deposited: | 16 Oct 2018 07:41 |
Last Modified: | 05 Aug 2021 08:08 |
URI: | http://repository.uph.edu/id/eprint/1372 |