Growth and fermentation conditions ofS.cerevisiae were optimized in this study using copper, manganese, and zinc supplementation into the growth medium. S.cerevisiae was cultured on two different basic media, i.e.molasses and soy bean sprouts extract with addition of ion metal (0,1 g/l of each) at different physical growth temperatures (27, 30, and 37°C) and pH values (4, 5, 6). The results indicated the optimum growth conditions were found at temperature 30°C and pH 5using molasses. Following the optimum growth conditions, fermentation was then performedon both semi-aerobic (shaker) and anaerobic (anaerobic jar) conditions for 8 h. The optimum aeration condition for fermentation was semiaerobic with resulted TPC value of 107 CFU/mland the alcohol concentration obtained was 10.5-11%. The biomass concentration under semi-aerobicconditions was 7,74-8,71 mg/ml, which was significantly higher than anaerobic (4095-4290 μg/ml). The Zn and Mn uptakes were 442.145 μg/g a