Mikroenkapsulasi lactobacillus plantarum F75 dan lactobacillus casei 97/L3 menggunakan natrium alginat-kitosan = Microencapsulation of L. Plantarum F75 and L. Casei 97/l3 in Sodium Alginate – Chitosan

Sthefanie, Sthefanie (2018) Mikroenkapsulasi lactobacillus plantarum F75 dan lactobacillus casei 97/L3 menggunakan natrium alginat-kitosan = Microencapsulation of L. Plantarum F75 and L. Casei 97/l3 in Sodium Alginate – Chitosan. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Pada penelitian kali ini, Lactobacillus plantarum F75 dan Lactobacillus casei 97/L3 dimikroenkapsulasi menggunakan natrium alginat (4%) serta kitosan (0,75%) melalui teknik ekstrusi. Mikrokapsul yang terbentuk kemudian di freeze dry untuk mengurangi konten air yang terkandung dan memperpanjang shelf-life. Suspensi sel dan mikrokapsul disimpan pada suhu kulkas (4°C) serta suhu ruang selama 28 hari. Setelah 28 hari, sel dan mikrokapsul yang menunjukkan stabilitas tinggi adalah yang disimpan pada suhu 4°C. Uji in vitro juga dilakukan pada simulated gastric juice (pH 2,0) dan intestinal juice (garam empedu) selama 120 menit pada suhu 37°C untuk mengetahui tingkat keselamatan probiotik bebas maupun yang dimikroenkapsulasi. Hasilnya menunjukkan bahwa mikroenkapsulasi menggunakan natrium alginat – kitosan sukses melindungi probiotik secara signifikan pada pH yang rendah, namun tidak pada lingkungan yang mengandung garam empedu

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Sthefanie, SthefanieNIM00000007502UNSPECIFIED
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorTan, Tjie JanNIDN8808640017UNSPECIFIED
Thesis advisorSugata, MarceliaNIDN0321118902UNSPECIFIED
Additional Information: SK 113-14 STH m 2018; 31001000176801
Uncontrolled Keywords: Alginate; sodium alginate; chitosan; Lactobacillus plantarum F75; Lactobacillus casei 97/L3
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology
Depositing User: Mrs Veronica Fitri Astuti
Date Deposited: 15 May 2021 14:40
Last Modified: 01 Nov 2021 08:16
URI: http://repository.uph.edu/id/eprint/26729

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