Latar Belakang: E-learning merupakan sistem pembelajaran sebagai sarana dalam proses belajar mengajar tanpa harus bertemu secara langsung. E-learning memengaruhi minat belajar mahasiswa karena adanya fasilitas seperti multimedia. Penelitian awal yang dilakukan oleh peneliti menunjukkan hasil bahwa mahasiswa tahun kedua tertarik menggunakan e-learning tetapi mereka berpendapat bahwa e-learning tidak meningkatkan minat belajar mahasiswa. Tujuan: Mengidentifikasi hubungan penerapan e-learning dengan minat belajar mahasiswa keperawatan di masa pandemi Covid-19. Metode: Peneliti menggunakan metode penelitian deskriptif korelasi. Populasi dalam penelitian ini 348 dengan sampel sebanyak 205 mahasiswa dan penelitian ini menggunakan metode purposive sampling. Analisis data yang digunakan adalah spearman rank. Hasil: Analisis spearman menunjukkan adanya hubungan yang kuat antara e-learning terhadap minat belajar dengan (nilai p = 0.001, rs = 0,705). Rekomendasi: Bagi peneliti selanjutnya disarankan membahas faktor lain yang memengaruhi minat belajar mahasiswa keperawatan / Background: E-learning is a learning system as a tool in teaching and learning process without having to meet face to face. E-learning affects students' interest in learning because of the facilities such as multimedia. Early research conducted by researchers shows the results that second year students are interested in using e-learning but they said that e-learning does not increase students' interest in learning. Objective: To identify the relationship between the application of e�learning and the learning interests of nursing students during the Covid-19 pandemic. Methods: Researchers used a correlation descriptive research method. The population in this study was 348 with a sample of 205 students and this study used a purposive sampling method. The data analysis used was the spearman rank. Results: Spearman analysis showed a strong relationship between e-learning and learning interest (p value = 0.001, rs = 0.705). Recommendation: It is advisable for further researchers to discuss other factors that affect the learning interest of nursing students.