Latar Belakang: Penanganan COVID-19 di seluruh dunia sangat berpengaruh terhadap keamanan petugas kesehatan. Profesi perawat selama masa pandemi coronavirus disease (COVID-19) mendapatkan tantangan untuk memberikan asuhan keperawatan yang profesional dengan resiko tertular bahkan menjadi korban COVID-19. Penanganan COVID-19 di seluruh dunia sangat berpengaruh terhadapa keamanan petugas kesehatan. Pemerintah menghimbau semua petugas kesehatan selalu menggunakan Alat Pelindung Diri (APD), menjaga jarak dan mencuci tangan. Tujuan Penelitian: Mengetahui hubungan tingkat pengetahuan dengan sikap karyawan dalam pencegahan COVID-19. Metode: Penelitian ini menggunakan penelitian deskriptif korelasional. Populasi penelitian ini adalah perawat yang bekerja di satu Rumah Sakit Swasta Indonesia Bagian Barat dengan riwayat pernah menjalani isolasi di Rumah Sakit yang berjumlah 35 perawat. Pengambilan sampel dalam penelitian ini menggunakan teknik total sampling. Hasil Penelitian : Hasil menunjukkan bahwa sebanyak 30 responden (96,9%) memiliki tingkat pengetahuan baik akan pencegahan penyebaran Covid-19 dan menunjukkan sikap yang positif. Sebanyak 2 responden (3,1%) memiliki pengetahuan yang cukup dan 1 responden menunjukkan sikap positif serta 1 responden lagi menunjukkan sikap negatif, sehingga didapatkan hasil bahwa tidak ada hubungan antara tingkat pengetahuan dengan sikap perawat terhadap pencegahan penularan Covid-19 di Rumah Sakit Swasta Bagian Barat./ Background: Handling COVID-19 greatly affects the safety of health workers around the world. The nursing profession is challenged during this coronavirus disease (COVID-19) pandemic to provide professional nursing care with the risk of contracting and even becoming victims of COVID-19. Handling COVID-19 around the world has important effect on health workers’ safety. The government urges to all Health Workers to always use Personal Protective Equipment (PPE), keep the distance and wash hand. Research Objectives: To determine the relationship between knowledge levels and employee attitudes in preventing COVID-19. Methods: This study uses descriptive correlation research. The population of this study were nurses who worked in a private hospital in the western part of Indonesia with a history of having been isolated in a hospital that collected 35 nurses. Sampling in this study using total sampling technique. Results : The results showed that as many as 30 respondents (96.9%) had a good level of knowledge about preventing the spread of Covid-19 and showed a positive attitude. As many as 2 respondents (3.1%) had sufficient knowledge, 1 respondent showed a positive attitude and 1 respondent showed a negative attitude, so the result was that there was no relationship between the level of knowledge and attitudes of nurses towards preventing Covid-19 transmission in one west private hospital.