Penelitian ini dilakukan untuk mengetahui pengaruh job stress terhadap public sevice motivation dan job performance. Sampel pada penelitian ini yaitu seluruh karyawan di RSU Bintang Semarapura Bali sebanyak 134 responden. Pengumpulan data dilakukan dengan menyebarkan kuesioner berisi 20 pertanyaan dengan Skala Likert 1 – 5 berdasarkan metode simple random sampling. Analisis data menggunakan metode SEM berbasiskan PLS. Hasil penelitian ini menyatakan bahwa challenge stress secara langsung berpengaruh positif dan signifikan terhadap job performance dan public service motivation. Namun hindrance stress secara langsung berpengaruh negatif dan signifikan terhadap job performance dan public service motivation. Sementara itu, public service motivation terbukti memediasi pengaruh positif antara challenge stress terhadap job performance dan secara negatif antara hindrance stress terhadap job performance./This research was conducted to determine the effect of job stress on public service motivation and job performance. The sample in this study were all employees at RSU Bintang in the Semarapura area of Bali as many as 134 respondents. The data was collected by distributing a questionnaire containing 20 questions with a Likert scale of 1 - 5 based on the simple random sampling method. Data analysis using PLS-based SEM method. The results of this study state that challenge stress has a positive and significant direct effect on work performance and motivation for public services. However, stress barriers directly have a negative and significant effect on work performance and motivation for public services. Meanwhile, public service motivation has been shown to mediate the positive effect of challenge stress on performance and negatively between obstacle stress on performance.