Background: Patient safety is a measure that is important from the quality of health services. The interview result obtained at least one until two patient safety incidents in a month as patient falls and giving the drug to the wrong patient. The behavior of the nurse in carrying out patient safety is influenced by the organizational culture, vision, mission, goals, and leadership style. The culture of the organization that are inherent in nurses affect the quality of hospital services and nurses can reflect itself as a professional nurse with the perform culture of the organization Objective: To determine the relationship between organizational culture with the behavior of the nurse in carrying out patient safety. Method: Using research methods correlational quantitative methods cross-sectional. The sample amounted to 55 nurses were taken using total sampling. Instruments in the form of questionnaires of organizational culture and the behavior of the nurse in carrying out patient safety that is propagated online through google forms and questionnaires directly to nurses. The questionnaire has been conducted to test the validity and reliability of the 31 nurses. The test results of the questionnaire of organizational culture with the count r 0,475-0,811 and the value of Cronbach alpha 0,962 while the questionnaire of nurse behavior with the count r 0,444-0,911 and value Cronhbach alpha 0,982. Analysis of data using the test of Spearman. The research started at 29 March-June 2020. Results: 58.2% the nurse perceives the organizational culture that is less, and 54,5% of nurses have poor behavior in implementing patient safety. The results of the test of Spearman showed an association (p=0.002) the culture of the organization with the behavior of the nurse in carrying out patient safety. Recommendation: The hospital can do coaching by the head nurse or team leader in the form of socialization, training, mentoring, and supervision.