Aktivitas antifungal Bacillus amyloliquefaciens N1 dan Lactobacillus fermentum E5 terhadap Aspergillus welwitschiae dan Penicillium sp. = Antifungal activity of Bacillus amyloliquefaciens N1 and Lactobacillus fermentum E5 against Aspergillus welwitschiae and Penicillium sp.

Subroto, Sara Thalia (2021) Aktivitas antifungal Bacillus amyloliquefaciens N1 dan Lactobacillus fermentum E5 terhadap Aspergillus welwitschiae dan Penicillium sp. = Antifungal activity of Bacillus amyloliquefaciens N1 and Lactobacillus fermentum E5 against Aspergillus welwitschiae and Penicillium sp. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Kontaminasi jamur memiliki dampak negatif dalam bidang industri dan kesehatan, khususnya jamur yang menghasilkan zat toksin. Pada umumnya, dalam mengatasi masalah kontaminasi jamur digunakan zat kimia yang berpotensi membahayakan kesehatan dan tidak ramah lingkungan. Oleh sebab itu, penggunaan agen biologis berupa bakteri yang memiliki sifat antifungal dapat menjadi solusi karena mudah ditemukan, lebih aman dan ramah lingkungan. Penelitian ini melakukan uji aktivitas antifungal dari Bacillus amyloliquefaciens N1 dan Lactobacillus fermentum E5 terhadap Aspergillus welwitschiae dan Penicillium sp melalui metode zona inhibisi dengan model whole cell. Selain itu, dilakukan juga analisis bioinformatika untuk mengetahui keberadaan gen yang terkait dengan komponen antifungal yang dihasilkan. Berdasarkan penelitian yang dilakukan pada uji aktivitas antifungal, B.amyloliquefaciens N1 dapat menghambat A. welwitchiae dan Penicillium sp. dengan persen inhibisi masing-masing adalah 73% dan 100%, sedangkan L. fermentum E5 tidak menunjukkan aktivitas antifungal terhadap kedua jamur. Pada analisis bioinformatika, komponen antifungal yang dimiliki B.amyloliquefaciens N1 diduga adalah bacillomycin D, surfactin dan fengycin. Dengan demikian, B. amyloliquefaciens N1 berpotensi untuk dikembangkan lebih lanjut dan dimanfaatkan terkait dengan sifat antifungal yang dimiliki. / Fungal contamination, especially by the toxin-producing fungi, has negative impacts on health and industry sectors. Chemicals are commonly used to eliminate the fungal contamination. However, those substances could be harmful for health and the environment. Therefore, the biological agent, such as bacteria able to inhibit fungal growth, would be a safer option. Hereby, a study on antifungal activity of Bacillus amyloliquefaciens N1 and Lactobacillus fermentum E5 against Aspergillus welwitschiae and Penicillium sp. was reported. The whole-cell method was used in this study. In addition, bioinformatic analysis was performed on whole genome of Bacillus amyloliquefaciens N1 to determine putative genes encoding antifungal compounds. Our result showed that Bacillus amyloliquefaciens N1 could inhibit Aspergillus welwitschiae and Penicillium sp. with the percent of inhibition were 73% and 100%. In contrast, Lactobacillus fermentum E5 was observed to exhibit no antifungal activity. Subsequently, the bioinformatic analyses indicated that the antifungal components of Bacillus amyloliquefaciens N1 were bacillomycin D, surfactin and fengycin. In conclusion, Bacillus amyloliquefaciens N1 has the potential to be further developed and utilized due to its antifungal properties.

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