Immobilization of Semi-Pure Intracelluler Chitinase Obtained from Providencia Stuartii in Calcium Alginate and Its Application for N-Acetylglucosamine Production = Imobilisasi Kitinase Semi Murni dari Bakteri Providencia Stuartii menggunakan Kalsium Alginat dan Aplikasinya dalam Produksi N-Asetilglukosamin

Hendarlim, Benedictus Dustin (2019) Immobilization of Semi-Pure Intracelluler Chitinase Obtained from Providencia Stuartii in Calcium Alginate and Its Application for N-Acetylglucosamine Production = Imobilisasi Kitinase Semi Murni dari Bakteri Providencia Stuartii menggunakan Kalsium Alginat dan Aplikasinya dalam Produksi N-Asetilglukosamin. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Chitin, a linear polymer of ß-1,4-N-acetylglucosamine units, is found naturally in shrimp shells and can be derived into glucosamine, which has a wide range of use, especially in the therapeutic field to treat joint damage. N-acetylglucosamine (NAG), one of the forms of glucosamine, can be produced by fermentation of chitin using chitinolytic microorganisms, such as mold or bacteria. Chitinase production by Providencia stuartii has been studied. However, immobilization of chitinase towards production of NAG has not been directly evaluated. This research was conducted to determine the effect of immobilized enzyme fermentation cycles on NAG production from chitin obtained from Penaeus monodon shells using Providencia stuartii. Mixture with enzyme volume of 2 ml and alginate volume of 1 ml was chosen to be used for the second stage research with a controlled pH of media at 5 for 6 hours at 40oC. Different fermentation cycles starting from 1 cycle, 2 cycles, 3 cycles and 4 cycles was chosen. The highest enzyme activity was produced by mixture with the enzyme volume of 2 ml and alginate volume of 1 ml with the enzyme activity of 2.030 ± 0.0405 U/ml. The most efficient cycles for NAG production was achieved from the first cycle, resulting in an average total NAG concentration of 1347.7778 ± 50.1848 ppm.

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