The purpose of this study was to examine and analyze the effect of high performance work system and emotional intelligence on employee well-being: perceived organizational support as a mediator for nurses at XYZ Hospital in South Jakarta. The method in this study is a quantitative study with a cross-sectional study design. The sampling method used was saturated sampling on 72 nurses at XYZ Hospital in South Jakarta, data collection using a questionnaire. Data analysis used a partial least squarestructural equation modeling approach with the SmartPls program. The results showed that the high performance work system (HPWS) had a positive effect on employee well-being, emotional intelligence (EI) had a positive effect on employee well-being, and perceived organizational support (POS) had a positive effect on employee wellbeing, high performance. work system (HPWS) has been shown to have a positive effect on perceived organizational support (POS). There are a number of limitations which are followed by suggestions for further research. /Tujuan penelitian ini untuk menguji dan menganalisis pengaruh pengaruh high performance work system dan emotional intelligence terhadap employee well-being: perceived organizational support sebagai mediator pada perawat RS XYZ di Jakarta Selatan. Metode dalam penelitian ini adalah penelitian kuantitatif dengan desain crosssectional study. Metode sampling yang digunakan sampling jenuh pada 72 perawat RS XYZ di Jakarta Selatan, pengumpulan data menggunakan kuesioner. Analisa data menggunakan pendekatan partial least square-structural equation modeling dengan program SmartPls. Hasil penelitian menunjukkan high performance work system (HPWS) terbukti berpengaruh positif terhadap employee well-being, emotional intelligence (EI) terbukti berpengaruh positif terhadap employee well-being, perceived organizational support (POS) terbukti berpengaruh positif terhadap employee wellbeing, high performance work system (HPWS) terbukti berpengaruh positif terhadap perceived organizational support (POS). Terdapat sejumlah keterbatasan yang diikuti saran bagi penelitian selanjutnya.