The in dubio pro natura principle is a principle that makes the rights of the environment must be prioritized regardless of whether the perpetrator of the crime can provide evidence in court. This principle is part of the precautionary principle in Act number 32 of 2009 concerning Environmental Protection and Management. This research uses normative juridical research method with statutory approach and case approach. From the results of the study it can be concluded that the in dubio pro natura principle which is part of the precautionary principle has been contained in environmental regulations in Indonesia and has been used as a consideration by judges in cases of environmental pollution committed by PT Kallista Alam.