Latar Belakang: Mencuci tangan menggunakan sabun merupakan upaya protokol kesehatan untuk mencegah penularan virus COVID-19. Terdapat penelitian yang menunjukkan adanya hubungan antara pengetahuan dan perilaku cuci tangan menggunakan sabun. Sementara, menurut laporan tingkat pengetahuan dan cuci tangan di Indonesia masih rendah, terbukti persentase pengetahuan dan perilaku cuci tangan yang benar di Jakarta Timur hanya 53,4%. Jakarta Timur merupakan daerah ibu kota yang sudah menerapkan proses pembelajaran tatap muka terbatas (PTMT) di masa pandemi dengan menerapkan perilaku baru di antaranya mencuci tangan, sehingga penelitian ini untuk mengetahui bagaimana hubungan pengetahuan dan perilaku cuci tangan pada PTMT siswa SD kelas 4-6 di masa pandemi COVID-19. Metode Penelitian: Penelitian ini merupakan tipe studi analitik komperatif kategorik tidak berpasangan dan desain studi kuantitatif potong lintang terhadap 104 responden yang memenuhi kriteria penelitian. Hasil: 71 responden memiliki pengetahuan yang baik dengan presentase 68,3%. Didapatkan 89 responden memiliki perilaku baik dengan presentase 85,6%. Hasil uji hubungan pengetahuan dan sikap yaitu nilai P 0,001, OR 8,375(CI: 2,420 – 28,987). Kesimpulan: Terdapat hubungan signifikan antara pengetahuan dan perilaku cuci tangan pada PTMT siswa SD kelas 4-6 di masa pandemi COVID-19. Kata Kunci: Mencuci tangan, Pembelajaran tatap muka terbatas, Pengetahuan, Perilaku, Siswa SD / Background: Washing hands with soap is an effort of health protocols to prevent the transmission of the COVID-19 virus. Correct hand washing behavior is based on good hand washing knowledge. There are studies that show a relationship between knowledge and behavior of washing hands using soap. Meanwhile, according to reports the level of knowledge and hand washing in Indonesia is still low, it is proven that the percentage of knowledge and correct hand washing behavior in East Jakarta is only 53.4%. East Jakarta is the capital city area that has implemented a limited face-to-face learning process (PTMT) during the pandemic by implementing new behaviors including washing hands, so this study is to find out how the knowledge and behavior in PTMT of elementary school students in grades 4-6 during the COVID-19 pandemic. Methods: This research is a type of unpaired categorical comparative analytic study and a cross-sectional quantitative study design with a sample of 104 students who met the inclusion criteria. Results: 71 respondents have good knowledge with a percentage of 68.3%. 89 respondents had good behavior with a percentage of 85.6%. The results of the test of the relationship between knowledge and attitudes were P value 0.001, OR 8.375 (CI: 2.420 – 28.987). Conclusion: There is a significant relationship between knowledge and hand washing behavior in PTMT elementary school students grades 4-6 during the COVID-19 pandemic. Keywords: Hand washing, Limited face-to-face learning, Knowledge, Behavior, Elementary school student.