Berdasarkan pra siklus yang dilakukan oleh peneliti terhadap siswa kelas X-1 SMA X di Toraja Utara ditemukan bahwa siswa kurang aktif dalam bertanya, menjawab, mendengarkan pendapat orang lain, dan mencatat rangkuman saat pembelajaran Biologi. Hal tersebut disimpulkan berdasarkan jurnal refleksi peneliti dan umpan balik mentor saat pra siklus. Oleh karena itu, peneliti memutuskan untuk menerapkan teknik bertanya guru untuk meningkatkan keaktifan belajar siswa dalam bertanya, menjawab, mendengarkan pendapat orang lain, dan mencatat rangkuman. Tujuan yang ingin dicapai melalui penelitian ini adalah mengetahui berhasil atau tidaknya teknik bertanya guru dan menjelaskan penerapan teknik bertanya guru untuk meningkatkan keaktifan belajar siswa kelas X-1 SMA X di Toraja Utara pada pembelajaran Biologi. Penelitian ini menggunakan metode Penelitian Tindakan Kelas (PTK) model Kemmis dan Taggart. Penelitian ini melalui tahap pra siklus dan tahap pelaksanaan berupa perencanaan, tindakan, observasi, dan refleksi. Penelitian ini dilaksanakan dalam tiga siklus yaitu pada tanggal 7 Oktober 2015, 21 Oktober 2015, dan 27 Oktober 2015. Peneliti menggunakan beberapa instrumen pada penelitian ini, yaitu lembar angket siswa, lembar observasi guru (mentor), lembar observasi teman sejawat dan lembar jurnal refleksi peneliti. Berdasarkan penelitian ini, peneliti menyimpulkan bahwa penerapan teknik bertanya mampu meningkatkan keaktifan belajar siswa dalam bertanya, menjawab, mendengarkan pendapat orang lain dan mencatat rangkuman. / According to the research that was done to grade X-1, SMA X in North Toraja, it was found that the students are lack of activeness in asking, answering, listening to other’s opinion and taking notes during Biology lesson delivery. This finding was concluded based on researcher’s reflection journal and mentor’s feedback when pre-cycle observation and teaching was done. Therefore, the researcher decided to implement teacher’s questioning technique to improve the students’ learning activeness in asking, answering, listening other’s opinion, and taking notes. The purposes of this research are to know the result of applying teacher’s questioning technique and to explain how to apply the teacher’s questioning technique to improve the learning activeness during Biology teaching and learning process of Grade X-1 students of SMA-X in North Toraja. Kemmis and Taggart’s model of Classroom Action Research was used in this research. Pre-cycle observation and teaching was included in this research and then it was continued by implementation stages: planning, acting, observing and reflecting, done in three cycles on October 7, 21 and 27, 2015. Researcher used Student’s Questionnaire sheet, Mentor’s Observation sheet, Peer Observation sheet and Researcher’s reflection journal as the research instruments. Based on this research, the researcher concluded that the implementation of questioning technique done by the teacher can improve the students’ learning activeness in asking, answering, listening other’s opinion and taking notes.