Malaria is a disease caused by Plasmodium transmitted through a vector called Anopheles mosquito. Until now research to create an effective vaccine against malaria has not yielded result. Moreover problem of resistance in the mosquito and the Plasmodium paratsite worsen the situation. Probiotic is a bacteria that when administered orally can bring beneficial properties to the host. Further, it is thought to act as an immunomodulator to combat malaria infection. The purpose of this study was to evaluate the potential of live cultures bacteria Pediococcus pentosaceus D32 collection of UPH in modulating the host immune system Balb/c mice to fight infection by Plasmodium berghei. The research utilized 65 male Balb/c mice 5 weeks old, which were divided into 5 groups: control with no treatment, placebo (PBS)+P. berghei, P. pentosaceus D32 + P. berghei, L. fermentum E5 + P. berghei (positive control), and P. berghei only. Spleen and blood serum harvested in H-0, H + 7, H + 14 and H + 21 after the injection of P. berghei were analyzed through phagocytosis test, hemagglutination test and measurement of the levels of TNF-alpha. Probiotic cultures administered orally for 14 days, before injected with 4 million by Plasmodium berghei ANKA. E5Pb & D32Pb groups have the highest percentage of inhibition in phagocytosis test on H+21 (74.71% & 74.91%). The highest antibody titer was found in group D32Pb at H+14 (log 2 titre 18.56). Interestingly, the highest concentrations of TNF-alpha was observed in H+7 in P.berghei control group (log 1.16) and the lowest in D32Pb group (0.52 log)