Perbandingan daya antiseptik ekstrak daun sirih (piper betle linn.) dengan ekstrak daun jambu biji (psidium guajava linn.) terhadap pertumbuhan koloni staphylococcus aureus dan eschericia coli = Antiseptical comparison between piper betle linn. and psidium guajava linn. extraction in growth of staphylococcus aureus and eschericia coli

Lie, Hendry (2018) Perbandingan daya antiseptik ekstrak daun sirih (piper betle linn.) dengan ekstrak daun jambu biji (psidium guajava linn.) terhadap pertumbuhan koloni staphylococcus aureus dan eschericia coli = Antiseptical comparison between piper betle linn. and psidium guajava linn. extraction in growth of staphylococcus aureus and eschericia coli. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Indonesia is a tropical country that has a very rich flora biodiversity and abundant availability. Many scientists are competing to empower these kinds of flora for the medical field. Among them are Betel Leaves and Guava which are very easy to find everywhere so there are many studies on the use of these leaves, one of which is both leaf extracts proven to be bactericidal because of the phenol, oil, and fatty acids contained in it so that both extracts can become an alternative to be used as an antiseptic raw material. The purpose of this study was to find out the antiseptic ability of Betel Leaf extract, Guava Leaf, or a mixture of both as an antiseptic by looking at its ability to inhibit the growth of Staphylococcus aureus and Eschericia coli bacteria. The test method used is the agar plate diffusion method where the Staphylococcus aureus and Eschericia coli media were observed inhibitory zones formed from leaf extracts with a concentration of 12.5%, 25%, 50%, positive control, and negative controls. The study was conducted from January to July 2018. Data processing used categorical and categorical bivariate analysis. It is proven that Betel Leaf Extract has the strongest antiseptic power compared to other extracts which are characterized by an average inhibition zone formed on the medium of Staphylococcus aureus and Eschericia coli greater than Guava extract and a mixture of Betel Leaf with Guava in various extract concentrations / Indonesia merupakan negara tropis yang memiliki keanekaragaman hayati flora yang sangat kaya dan ketersediaan yang sangat melimpah. Banyak ilmuan berlomba-lomba untuk memberdayakan macam-macam flora tersebut untuk bidang medis. Diantaranya ialah Daun Sirih dan Jambu Biji yang sangat mudah ditemui dimana-mana sehingga banyak penelitian tentang pemanfaatan daun tersebut, salah satunya ialah kedua ekstrak daun tersebut terbukti bersifat bakterisid karena kandungan fenol, minyak, dan asam lemak yang terkandung didalamnya sehingga harusnya kedua ekstrak tersebut dapat menjadi alternatif untuk dijadikan bahan baku antiseptik. Tujuan penelitian ini adalah untuk mencari tahu kemampuan antiseptik dari ekstrak Daun Sirih, Daun Jambu Biji, atau campuran keduanya sebagai antiseptik dengan melihat kemampuannya menghambat pertumbuhan bakteri Staphylococcus aureus dan Eschericia coli. Metode pengujian yang digunakan ialah metode difusi lempeng agar dimana media Staphylococcus aureus dan Eschericia coli diamati zona hambat yang terbentuk dari ekstrak daun dengan konsentrasi 12.5%, 25%, 50%, kontrol positif, dan kontrol negatif. Penelitian dilakukan pada bulan Januari hingga Juli 2018. Pengolahan data menggunakan analisis bivariat kategorik dan kategorik. Terbukti bahwa Ekstrak Daun Sirih memiliki daya antiseptik paling kuat dibandingkan ekstrak lainnya yang ditandai dengan rata-rata zona hambat yang terbentuk pada media Staphylococcus aureus dan Eschericia coli lebih besar dibandingkan ekstrak Jambu Biji dan campuran Daun Sirih dengan Jambu Biji di macam-macam konsentrasi ekstrak.

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