UJI AKRIVITAS EKSTRAK ETANOL DAUN DAN RANTING Artabotrys sumatranus TERHADAP JAMUR Candida albicans

Iskandar, Gibran Naufan (2025) UJI AKRIVITAS EKSTRAK ETANOL DAUN DAN RANTING Artabotrys sumatranus TERHADAP JAMUR Candida albicans. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Candida albicans merupakan salah satu jamur pada genus Candida yang dapat menyebabkan infeksi pada manusia. Jamur ini bersifat opurtunistik, dengan kata lain pertumbuhan Candida albicans yang berlebih pada tubuh manusia disebabkan oleh penurunan sistem kekebalan tubuh. Candida albicans umum ditemukan pada bagian tubuh manusia, terutama pada rongga mulut, lapisan kulit, saluran pencernaan, dan vagina. Pada ekstrak etanol daun dan ranting Artabotrys sumatranus telah diketahui memiliki kandungan senyawa utama yaitu mangiferin yang termasuk ke dalam senyawa golongan polifenol. Ada pun senyawa lainnya yang terkandung dalam ekstrak etanol daun dan ranting Artabotrys sumatranus adalah terpenoid, flavonoid, dan fenolik. Berdasarkan penelitian yang ada, mangiferin, terpenoid, flavonoid, dan fenolik telah terbukti dapat menghambat pertumbuhan jamur terutama terhadap Candida albicans, sehingga ekstrak daun dan ranting Artabotrys sumatranus sangat berpotensi untuk digunakan sebagai agen antijamur terutama terhadap Candida albicans. Ekstraksi dilakukan menggunakan pelarut etanol absolut dengan perbandingan 1:5 (serbuk Artabotrys sumatranus : etanol absolut) dengan metode maserasi selama satu hari (24 jam) dan dilakukan remaserasi selama 24 jam dengan menggunakan pelarut serta perbandingan yang sama. Aktivitas antijamur diamati melalui pengujian Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) dengan menggunakan metode dilusi. Hasil KHM dan KBM menunjukkan bahwa ekstrak daun dan ranting Artabotrys sumatranus dapat menghambat serta membunuh Candida albicans dengan nilai persen penghambatan mencapai 97,7737% pada ekstrak daun dan 98,3115% pada ekstrak ranting. Nilai KHM ekstrak daun adalah 2,5 mg/mL, sedangkan ekstrak ranting adalah 15 mg/mL. Nilai KBM ekstrak daun adalah 5 mg/mL, sedangkan ekstrak ranting adalah 15 mg/mL. / Candida albicans is a fungus in the genus Candida that can cause infections in humans. This fungus is opportunistic, in other words the excessive growth of Candida albicans in the human body is caused by a decrease in the immune system. Candida albicans is commonly found in parts of the human body, especially in the oral cavity, skin lining, digestive tract and vagina. The ethanol extract of Artabotrys sumatranus leaves and grubs is known to contain the main compound, namely mangiferin, which is included in the polyphenol group of compounds. There are also other compounds contained in the ethanol extract of Artabotrys sumatranus leaves and grubs, namely terpenoids, flavonoids and phenolics. Mangiferin, terpenoids, flavonoids, and phenolics have been proven to inhibit fungal growth, especially against Candida albicans, so that leaf extract and grumble Artabotrys sumatranus are very likely to be used as antifungal agents, especially against Candida albicans. Extraction was carried out using ethanol pro analysis solvent with a ratio of 1:5 (Artabotrys sumatranus powder: ethanol pro analysis) using the maceration method for one day (24 hours) and remaceration was carried out for 24 hours using the same solvent and ratio. Antifungal activity was observed by testing the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MBC) using the dilution method. The results of MIC and MFC showed that the extract of Artabotrys sumatranus leaves and twigs could inhibit and kill Candida albicans with inhibition percentage values reaching 97.7737% in the leaf extract and 98.3115% in the twig extract. The MIC value of the leaf extract is 2.5 mg/mL, while the twig extract is 15 mg/mL. The MBC value of the leaf extract is 5 mg/mL, while the twig extract is 15 mg/mL.
Item Type: Thesis (Bachelor)
Creators:
Creators
NIM
Email
ORCID
Iskandar, Gibran Naufan
NIM01038200042
gibrannaufan01@gmail.com
UNSPECIFIED
Contributors:
Contribution
Contributors
NIDN/NIDK
Email
Thesis advisor
Rosa, Dela
NIDN0312127802
dela.rosa@uph.edu
Thesis advisor
Pranasti, Ernestine Arianditha
NIDN0307119603
ernestine.pranasti@uph.edu
Uncontrolled Keywords: Artabotrys sumatranus; mangiferin; Candida albicans; antijamur
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Health Sciences > Pharmacy
Current > Faculty/School - UPH Karawaci > Faculty of Health Sciences > Pharmacy
Depositing User: Gibran Naufan Iskandar
Date Deposited: 28 Jul 2025 04:19
Last Modified: 28 Jul 2025 04:19
URI: http://repository.uph.edu/id/eprint/70141

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