Reduksi logam berat cumi-cumi, kerang hijau, udang jerbung menggunakan kombinasi Saccharomyces cerevisiae dan Candida albicans = Reduction of heavy metals in squid, green mussels, white shrimp using a combination of saccharomyces cerevisiae and candida Albicans

Wijaya, Trifena (2020) Reduksi logam berat cumi-cumi, kerang hijau, udang jerbung menggunakan kombinasi Saccharomyces cerevisiae dan Candida albicans = Reduction of heavy metals in squid, green mussels, white shrimp using a combination of saccharomyces cerevisiae and candida Albicans. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Wilayah perairan Muara Angke, perairan Cituis di Tangerang, dan perairan Indramayu ditemukan berbagai kegiatan, seperti industri, tempat pelelangan ikan, dan pelabuhan kapal yang menjadi sumber cemaran logam berat. Pencemaran logam berat di laut akibat limbah industri dapat mengganggu biota laut yang hidup di perairan tersebut. Biota laut seperti cumi-cumi, kerang hijau, dan udang jerbung dapat mengakumulasi logam berat di dalam tubuhnya. Bioremediasi merupakan proses yang dilakukan untuk mengurangi logam berat secara biologis menggunakan mikroorganisme. Mikroorganisme yang dapat digunakan sebagai agen bioremediasi adalah khamir Sacchromyces cerevisiae dan Candida albicans yang diperoleh dari Indonesia Culture Collection (InaCC), LIPI Cibinong. Kombinasi khamir S. cerevisiae dan C. albicans dapat meningkatkan kadar reduksi logam berat. Tujuan penelitian ini adalah untuk menentukan kombinasi khamir terbaik dalam mereduksi kandungan logam berat dalam sampel. Pada penelitian pendahuluan, lokasi pengambilan sampel cumi-cumi, kerang hijau, dan udang jerbung menjadi faktor, untuk menentukan kadar logam tertinggi. Pada penelitian utama, perlakuan kombinasi khamir menjadi faktor dengan sembilan varisi level, untuk dilihat penuruan logam berat dalam sampel. Analisis kandungan logam berat dalam sampel dianalisis menggunakan Atomic Absoption Spectrophotometer (AAS). Hasil penelitan pendahuluan menunjukkan adanya pengaruh signifikan antara lokasi pengambilan sampel terhadap kandungan logam berat dalam sampel. Hasil penelitan utama menunjukkan bahwa perlakuan kombinasi khamir S. cerevisiae : C. albicans (60% : 40%) memberikan hasil penurunan logam berat terbaik dan memiliki jumlah sel khamir resisten logam berat tertinggi. / The area of Muara Angke waters, Cituis waters in Tangerang, and Indramayu waters found various activities, such as industry, fish auction sites, and ship ports which are sources of heavy metal contamination. Pollution of heavy metals at sea due to industrial waste can disrupt marine life that lives in these waters. Marine biota such as squid, green mussels and white shrimp can accumulate heavy metals in their bodies. Bioremediation is a process carried out to reduce heavy metals biologically using microorganisms. Microorganisms that can be used as bioremediation agents are yeast Sacchromyces cerevisiae and Candida albicans obtained from the Indonesian Culture Collection (InaCC), LIPI Cibinong. Yeast combination of S. cerevisiae and C. albicans can increase levels of heavy metal reduction. The purpose of this study is to determine the best yeast combination in reducing the content of heavy metals in the sample. In a preliminary study, the location of squid, green mussel and white shrimp sampling was a factor, to determine the highest metal content. In the main study, the treatment of the yeast combination was a factor with nine level variations, to see the decline of heavy metals in the sample. Analysis of heavy metal content in samples was analyzed using the Atomic Absorption Spectrophotometer (AAS). Preliminary research results indicate a significant effect between the sampling locations on the content of heavy metals in the sample. The main research results showed that the treatment of S. cerevisiae : C. albicans yeast (60% : 40%) combination gave the best weight loss results and had the highest number of resistant heavy metals yeast cells.

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