Kebutuhan perawatan paliatif terus meningkat di Indonesia, namun ketersediaan fasilitas perawatan paliatif masih sangat terbatas. Perawat di Puskesmas berperan dalam memberikan layanan perawatan paliatif terutama bagi pasien rawat jalan dan pengetahuan dari perawat tentang perawatan paliatif menjadi landasan penting dalam memberikan asuhan keperawatan. Tujuan penelitian ini adalah untuk mengetahui faktor-faktor yang berhubungan dengan pengetahuan perawat tentang perawatan paliatif di Puskesmas Kecamatan di DKI Jakarta. Pengetahuan perawat diteliti dengan menggunakan kuisioner Palliative Care Knowledge Test (Nakazawa, 2009). Penelitian ini menggunakan desain penelitian kuantitatif korelasi dengan metode cross-sectional dan teknik sampling accidental sampling dengan total sampel sebanyak 39 perawat. Hasil penelitian menunjukan rata-rata pengetahuan perawat adalah 32,82%. Tidak ada hubungan signifikan (p value > 0,05) antara pendidikan (p value 1,000), paparan media massa (p value 0,677) dan pengalaman perawat (p value 0,517) dengan pengetahuan perawat tentang perawatan paliatif. Namun berdasarkan variabel pendidikan perawat, paparan media massa dan pengalaman perawat ditemukan nilai yang berbanding lurus dengan pengetahuannya. Saran peneliti agar perawat terus aktif dalam memperkaya pengetahuan tentang paliatif melalui media massa dan Puskesmas dapat menyelenggarakan kegiatan-kegiatan untuk meningkatkan pengetahuan perawat tentang perawatan paliatif / Diseases that require palliative care continually increasing in Indonesia, but the availability of facilities for palliative care are still very limited. Nurses in health center play a role in providing palliative care service, especially for outpatient and knowledge of nurses about palliative care is an important basic for providing nursing care. The purpose of this study was to determine the factors associated with nurses' knowledge of palliative care in district health center in DKI Jakarta. Knowledge of nurses were measured with Palliative Care Knowledge Test (PCKT) questionnaire by Nakazawa (2009). This study used quantitative correlation design with cross-sectional method and accidental sampling technique with a total sample of 39 nurses. Study showed that an average knowledge of Health District nurses were 32.82%. There was no significant correlation (p value > 0.05) between the Nurses’ Education (p value 1.000), Mass Media Exposure (p value 0.677) and Nurses’ Experiences (p value 0.517) with their knowledge about palliative care. However, nurses education, mass media exposure and nurses’ experiences are directly proportional to knowledge of nurses. Researcher suggests that nurses at health center should actively enrich their knowledge of palliative care through the mass media and health centers could provide seminars, trainings or activities that are related to palliative care for nurses.