Some researches showed that black tiger prawn shell is a good source of carotenoids and the major carotenoids component from black tiger prawn shell is astaxanthin which is known to have a high antioxidant activity. Black tiger prawn shell commonly found from frozen prawn industries in Indonesia, however the study about carotenoid activity is still rare. The objective of this research is to characterize the carotenoid extract activity from black tiger prawn shell as antioxidant and antibacterial agent. In this research, carotenoid was extracted using three different temperatures (30oC, 45oC, 60oC) from three different solvents which were n-hexane, isopropyl alcohol, and mixture between n-hexane and isopropyl aclcohol. The extract yield, total carotenoid content, antioxiant activity (measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method), antibacterial activity (measured by well-diffusion agar method), and terpenoid content (measured by Liebermann-Burchard method) from the carotenoid extract were measured. Result showed that 30oC was the best temperature to extract carotenoid with the highest yield (0,076% in n-hexane; 1,51% in isopropyl aclcohol; 1,48% in mixture between n-hexane and isopropyl alcohol) and carotenoid content (1,48 µg/g in n-hexane; 3,16 µg/g in isopropyl alcohol; 3,71µg/g in mixture between n-hexane and isopropyl alcohol) from each solvent used. The mixture between n-hexane and isopropyl alcohol was the best solvent used to extract carotenoid with the highest in total carotenoid content (3,71 µg/g), antioxidant activity (IC50 of 2890 ppm), and terpenoid content. There was no antibacterial activity from the carotenoid extract to inhibit the growth of Escherichia coli (Gram negative bacteria) and Bacillus cereus (Gram positive bacteria).