Febriani, Enjelia (2022) Pengujian potensi aktivitas fibrinolitik bakteri yang diisolasi dari rusip = Examining potential fibrinolytic activity of bacteria isolated from rusip. Bachelor thesis, Universitas Pelita Harapan.
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Abstract
Penyakit kardiovaskuler menjadi penyebab utama kematian secara global, salah
satunya adalah terjadinya penggumpalan darah yang menyumbat aliran darah.
Terapi dengan obat antikoagulan oral yang menjadi pilihan utama masih memiliki
kekurangan terkait efek samping dan harga obat yang tergolong mahal. Oleh
karena itu, perlu dikembangkan pengobatan alternatif yang tergolong murah dan
aman, yaitu enzim yang bersifat fibrinolitik dari bakteri pada rusip, produk
fermentasi ikan teri (Stolephorus sp) asal Bangka Belitung. Dalam makanan
fermentasi yang terbuat dari Stolephorus sp sering ditemukan Bacillus subtilis.
Enzim ekstraselulernya diketahui mempunyai potensi fibrinolitik. Oleh karena itu,
diperlukan studi lebih lanjut sifat fibrinolitik tersebut. Perlu dicari bakteri yang
memiliki sifat proteolitik dan selanjutnya diuji fibrinolitik dari bakteri tersebut
yakni uji degradasi gumpalan darah, degradasi gumpalan euglobulin, dan
degradasi fibrin. Selanjutnya, identifikasi bakteri menurut flowcharts Bergey’s
Manual untuk mengetahui jenis bakteri yang didapatkan. Hasil pengujian
menunjukkan bahwa kedelapan protein sampel dari bakteri rusip mempunyai
potensi yang bersifat fibrinolitik dalam mendegradasi gumpalan darah, beberapa
pita komponen penyusun fraksi euglobulin, dan degradasi fibrin secara langsung.
Degradasi pita fraksi euglobulin dan fibrin divisualisasi melalui SDS-PAGE.
Berdasarkan pada hasil identifikasi bakteri, kedelapan bakteri diprediksi berasal
dari spesies Bacillus subtilis. / Cardiovascular disease is the main cause of death globally, one of which is blood
clots blocking blood flow. Oral anticoagulant drugs as the main choice for therapy
still have drawbacks related to the side effects and the price of drugs is quite
expensive. Therefore, an alternative relatively cheap and safe treatment needs to
be developed, namely fibrinolytic enzyme from bacteria in rusip, a fermented
product of anchovy (Stolephorus sp) from Bangka Belitung. In fermented foods
made from Stolephorus sp, Bacillus subtilis is often found. Its extracellular
enzymes are known to have great potential for fibrinolytic activity. Therefore,
further studies of fibrinolytic properties are needed. It is necessary to look for
bacteria that have proteolytic properties and then test for fibrinolytic from these
bacteria, namely blood degradation tests, euglobulin degradation, and fibrin
degradation. Next, identify bacteria according to Bergey's Manual flowcharts to
determine the type of bacteria obtained. The test results showed that the eight
sample proteins from rusip bacteria had fibrinolytic potential in degrading blood
clots, several bands of components of the euglobulin fraction, and direct fibrin
degradation. The band degradation of the euglobulin and fibrin fractions was
visualized using SDS-PAGE. According to the result of bacteria identification, the
eight bacteria are predicted belong to the Bacillus subtilis species.
Item Type: | Thesis (Bachelor) |
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Creators: | Creators NIM Email ORCID Febriani, Enjelia NIM01113180027 enjeliafebriani2@gmail.com UNSPECIFIED |
Contributors: | Contribution Contributors NIDN/NIDK Email Thesis advisor Pinontoan, Reinhard NIDN0325046502 reinhard.pinontoan@uph.edu Thesis advisor Kiranadi, Bambang NIDN0026125004 bambang.kiranadi@lecturer.uph.edu |
Uncontrolled Keywords: | Bacillus subtilis; degradasi fibrin; fibrinolitik; identifikasi; proteolitik; rusip; Bacillus subtilis; fibrin degradation; fibrinolytic; identification; proteolytic; rusip |
Subjects: | Q Science > Q Science (General) Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology |
Divisions: | University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Biology |
Depositing User: | Enjelia Febriani |
Date Deposited: | 25 Aug 2022 10:30 |
Last Modified: | 25 Aug 2022 10:30 |
URI: | http://repository.uph.edu/id/eprint/50036 |