Perbandingan aktivitas antimikrobial Lactiplantibacillus plantarum SU-KC1a dan F75 terhadap pertumbuhan bakteri patogen = Comparison of Lactiplantibacillus plantarum SU-KC1a and F75 antimicrobial activity against the growth of pathogenic bacteria

Widjaja, Anasthasia Nathania (2022) Perbandingan aktivitas antimikrobial Lactiplantibacillus plantarum SU-KC1a dan F75 terhadap pertumbuhan bakteri patogen = Comparison of Lactiplantibacillus plantarum SU-KC1a and F75 antimicrobial activity against the growth of pathogenic bacteria. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Bakteri asam laktat (BAL) memiliki peran yang penting dalam industry makanan, terutama BAL yang menghasilkan bakteriosin, yang digunakan sebagai kultur bioprotektif untuk meningkatkan kualitas dan keamanan pangan. Tidak hanya itu, produksi senyawa antimikroba oleh BAL probiotik penting bagi interaksi in vivo yang terjadi di dalam saluran gastrointestinal, yang juga berkontribusi terhadap kesehatan saluran penernaan. Penelitian secara in silico terhadap L. plantarum SU-KC1a, dengan metode anotasi genom, telah menunjukkan bahwa strain L. plantarum ini memiliki 15 gen pengode plantarisin. Meskipun demikian, aktivitas antimikroba dari L. plantarum SU-KC1a masih butuh dibuktikan secara wet lab. Oleh karena itu, penelitian ini dilakukan untuk menjelaskan aktivitas antimikroba dari L. plantarum SU-KC1a dan L. plantarum F75, yang sebelumnya sudah diteliti, terhadap E. coli, S. aureus, dan L. monocytogenes. Hasil dari penelitian ini menunjukkan bahwa supernatan dari L. plantarum SU-KC1a dan F75 memiliki aktivitas antimikroba terhadap E. coli dan S. aureus. Namun, supernatan dari kedua L. plantarum memiliki efek yang terbalik terhadap L. monocytogenes, yang ditumbuhkan pada TSA. Supernatan L. plantarum tampak malah menunjang pertumbuhan L. monocytogenes. Berdasarkan uji-uji yang sudah dilakukan, disimpulkan bahwa aktivitas antimikroba dari kedua L. plantarum dikarenakan pH yang asam. / Lactic acid bacteria (LAB) have such an important role within the food industry, especially bacteriocin-producing LABs, as bioprotective culture to increase food quality and safety. Not only that, antimicrobial substance production by probiotic LABs is important for in vivo interaction that happens within the gastrointestinal tract, which also contributes to gut health. Previous in silico study on L. plantarum SU-KC1a genome by gene annotation, has found that this strain of L. plantarum has 15 plantaricin-coding gene. However, the antimicrobial activity of L. plantarum SU-KC1a still needs to be proven by wet lab. Therefore, this study is done to elucidate the antimicrobial activity of L. plantarum SU-KC1a and L. plantarum F75, which previously had been studied, against E. coli, S. aureus, and L. monocytogenes. The results show that supernatants of L. plantarum SU-KC1a and F75 both have antimicrobial activity against E. coli and S. aureus. However, the supernatants of both L. plantarum have a reverse effect of L. monocytogenes, which was grown on TSA, as it boosts the latter’s growth. Based on the assays done, the antimicrobial activity of both L. plantarum was due to acidic pH.

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