Stability of crude extract antioxidant from red dragon fruit (hylocereus polyrhizhus) peel toward ph and temperature = Stabilitas ekstrak kasar antioksidan dari kulit buah naga merah (hylocereus polyrhizus) terhadap ph dan suhu

Marcelia, Marcelia (2011) Stability of crude extract antioxidant from red dragon fruit (hylocereus polyrhizhus) peel toward ph and temperature = Stabilitas ekstrak kasar antioksidan dari kulit buah naga merah (hylocereus polyrhizus) terhadap ph dan suhu. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Red Dragon Fruit (Hylocereus polyrhizus) peel as a waste had not been optimally utilized. It contained antioxidant compound for example, phenolic compound. The purpose of this research is to study the stability of the antioxidant activity toward pH and temperature. The treatment in this study is conducted to determine the selected extract using combination of solvent ethanol and ethyl acetate with ratio 100:0, 80:20, 60:40, 40:60, 20:0, 0:100. Beside, the extraction is done by maceration with shaker using variation of temperature (26oC, 40oC, and 50oC) and time (3 hours, 6 hours, and 9 hours). After the best extract is obtained then continued with the use of pH and temperature by RSM. The pH was 4, 5, 6, 7, and 8 with 65oC, 75oC, 85oC, 95oC of heating in 30 minutes. The result showed that the combination of ethanol and ethyl acetate with ratio 60:40, 26oC of extraction temperature, and 6 hours of maceration had the best phenolic content and antioxidant activity. The best extract had phenolic content of 13.31 mg GAE/ g extract and IC50 of 603.80 ppm. pH and temperature can reduce antioxidant activity of red dragon fruit peel. Stability of antioxidant from red dragon peel obtained from pH 3.5 to 5.5 and temperature from 50oC to 75oC. The best antioxidant activity was obtained from extract with pH 4.44 and 62.7oC treatment. The best extract had phenolic content of 10.10 mg GAE/g extract and IC50 of 646.96 ppm of DPPH.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Marcelia, MarceliaNIM03420070113UNSPECIFIED
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorHardoko, HardokoNIDN9903005173UNSPECIFIED
Thesis advisorSiregar, Tagor M.NIDN0304016902UNSPECIFIED
Uncontrolled Keywords: Hylocereus polyrhizus; dragon fruit peel; antioxidant; pH; temperature; RSM
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Mrs Marselita Harapan
Date Deposited: 16 Oct 2018 07:35
Last Modified: 05 Aug 2020 03:47
URI: http://repository.uph.edu/id/eprint/1176

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