Isolasi dan identifikasi senyawa antimikroba kulit manggis (garcinia mangostana l.)

Pradina, Aurelia Aileen (2013) Isolasi dan identifikasi senyawa antimikroba kulit manggis (garcinia mangostana l.). Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Mangosteen peel has bioactive compounds that can inhibit antimicrobial activities but further information about its specific compounds and the antimicrobial activity changes between crude extracts and isolate had not been studied yet. Crude extracts of mangosteen peel in this experiment was tested for phytochemical compounds and antimicrobial activity. In order to isolate and identify antimicrobial compounds, thin layer chromatography, column chromatography, and gas chromatography were applied. Mobile phase for column chromatography is methanol:ethyl-acetate:chloroform in 1:1:8 ratio from prism method. This ratio produced 8 fractions in coloum chromatography. The maximum wavelengths for all fractions were scanned and all concentrated fractions used for antimicrobial activity test. The result showed the best inhibition zone came from F8 fraction. F8 fraction could inhibit Listeria monocytogenes, Enterobacter aerogenes, Escherichia coli but had no effect for Salmonella typhi, Penicillium sp., and Aspergillus niger. For the next step, F8 fraction was identified with GC-MS resulted in 51 compounds but there was 13 compounds with quality ≥ 95% as antimicrobial activity.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Pradina, Aurelia AileenNIM03420090020AILEEN_MANUTD@YAHOO.COM
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorParhusip, Adolf Jan NexsonNIDN0107016701UNSPECIFIED
Thesis advisorEveline, EvelineNIDN0308048201UNSPECIFIED
Uncontrolled Keywords: antimicrobial ; column chromatography ; isolation ; mangosteen peel
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Users 17 not found.
Date Deposited: 16 Oct 2018 07:36
Last Modified: 14 Oct 2020 05:02
URI: http://repository.uph.edu/id/eprint/1279

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