Isolation, characterization, and bioactivity assay of crude beta glucan from silver ear mushroom (tremella fuciformis)

Tjahja, Isaura Petrina (2012) Isolation, characterization, and bioactivity assay of crude beta glucan from silver ear mushroom (tremella fuciformis). Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Beta-glucan is a polysaccharide derived from mushrooms’ cell wall mainly used for increase the immune system. Besides, beta-glucan is also used to reduce the blood glucose level which can be done by doing beta-glucosidase inhibition test. This research was aimed to isolate and characterize crude beta-glucan from Tremellafuciformis,as well as assess its bioactivity. Hexane and ethyl acetate were used to remove impurities prior to hot water extraction to get the isolated beta-glucan, resulting in 2.580.33%. C-NMR, H-NMR, and FT-IR were used to confirm the characteristic of crude beta-glucan. C-H and CH2 groups were detected within δH 3.5-4.2 ppm, shown by H-NMR. Meanwhile anomeric proton that indicated β-glycosidic linkage was shown at 4.8-5.2 ppm, which was also confirmed by FT-IR in 1028 cm-1 region. The most abundant spectra shown by CNMR in chemical shift δC 71.58; 71.87; 72.90; 73.08; 73.47; 76.53; followed by δC 60.41; 60.95; indicated CH and CH2.Anomeric carbon that indicated glycosidic carbon was shown at 93.13-102.01 ppm. FT-IR showed the OH group at 3500-3000 cm-1, followed by 1250-1122 cm-1 for –C-O and 1500-1400 cm-1 for C-H absorption. In addition, methyl group which belong to fucose was shown by δH at 1.07 and 1.21 ppm which also conformed by the result of GC-MS showed the crude beta-glucan contained glucose, fucose, mannose, melibiose, and Dgalactose. Antidiabetic activity was measured by performing alpha-glucosidase inhibitory evaluation. Crude beta-glucan was found to have a weak activity on alpha-glucosidase since it has lower inhibitory activity than quercetin. The crude beta- glucan started to show its inhibitory activity at 50 ppm by resulting yellow transparent color with the highest activity of 41.612.25%, i.e. lower than quercetin as the positive control (71.410.25%).

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Tjahja, Isaura PetrinaNIM03420080067ISAURAIZHERE@YAHOO.COM
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorKardono, Leonardus Broto SugengUNSPECIFIEDUNSPECIFIED
Thesis advisorHalim, Jeremia ManuelNIDN0312118501UNSPECIFIED
Uncontrolled Keywords: beta-glucan; Tremella fuciformis; NMR; FT-IR, GC-MS; alpha-glucosidase inhibitior
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Mrs Marselita Harapan
Date Deposited: 16 Oct 2018 07:57
Last Modified: 03 Sep 2020 03:49
URI: http://repository.uph.edu/id/eprint/1703

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