Extraction and characterization of collagen from narrow-barred spanish mackerel (scomberomorus sp.) = Ekstraksi dan karakterisasi kolagen dari tulang ikan tenggiri (scomberomorus sp.)

Sari, Novel (2011) Extraction and characterization of collagen from narrow-barred spanish mackerel (scomberomorus sp.) = Ekstraksi dan karakterisasi kolagen dari tulang ikan tenggiri (scomberomorus sp.). Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Extraction and characterization of both collagen dialysis and collagen wash from the bone of narrow-barred Spanish mackerel were studied. Extraction method for both collagens differed in the purification process. Extraction has done by using NaOH to remove non-collagenous protein, 10% alcohol to remove fat, sodium-EDTA to remove mineral, 0.5 M acetic acid to dissolve collagen and NaCl to precipitate the collagen. The yields for both collagen dialysis and collagen wash are 1.91% and 2.06%, on the basis of lyophilized dry weight, respectively. The protein content of both collagen dialysis and collagen washare 83.00% and 48.98%, respectively. Collagen dialysis has higher water absorption, oil absorption and degree of whiteness compared to collagen wash, but has a lower bulk density than collagen wash. Collagen dialysis and collagen wash perfoms the highest solubility at pH 3 and concentration of 1% NaCl. Thermal denaturation of narrow-barred Spanish mackerel bone collagen ranged from 25 - 30°C. Increasing the temperature to more than 25 °C causes a decrease in viscosity.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Sari, NovelUNSPECIFIEDUNSPECIFIED
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorSuhartono, Maggy ThenawidjajaUNSPECIFIEDUNSPECIFIED
Thesis advisor., NataniaUNSPECIFIEDUNSPECIFIED
Uncontrolled Keywords: narrow-barred Spanish mackerel; collagen; extraction; characterization
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Mrs Marselita Harapan
Date Deposited: 16 Oct 2018 08:03
Last Modified: 02 Dec 2019 02:10
URI: http://repository.uph.edu/id/eprint/1829

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