Study of extraction and isolation of antioxidant from edible bird’s nest = Kajian ekstraksi dan isolasi antioksidan sarang burung walet

Januarti, Winny (2012) Study of extraction and isolation of antioxidant from edible bird’s nest = Kajian ekstraksi dan isolasi antioksidan sarang burung walet. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Edible bird’s nest is one of several animal products that is known for its function such as it can cure a cough, alleviate asthma, increase concentration, and recently research has been revealed that edible bird’s nest has antioxidant activity but the extraction, isolation and identification of antioxidant compounds have not been carried out. This research is designed to study extraction and isolation of antioxidant from edible bird’s nest. Edible bird’s nest is extracted using gradual maceration method with three different solvents; n-hexane, ethyl acetate, and ethanol. The isolation is done by Column Chromatography and Thin Layer Chromatography (TLC). This assay indicates that the crude extract is obtained by ethanol extract with IC50 43636,49 ppm. Maximum wavelengths of the ethanol extract are 315-696 nm. The phytochemical screening of ethanol extract shows the existence of alkaloids, flavonoids, tannins, and terpenoids. Fraction that is chosen and obtained from Column Chromatography is fraction B (80% ethyl acetate : 20% ethanol and 10% ethyl acetate and 90 ethyl acetate) with IC50 355,82 ppm. This assay indicates that fraction has lower IC50 than the crude extract. Fraction B is isolated with TLC and the best combination of solvents to get one spot is 20% ethyl acetate : 80% ethanol with Rf 0,82. Azulene, benzothiazole, hexadecane, and heptadecane as the isolated spot from TLC are investigated by GC-MS and show antioxidant activities.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Januarti, WinnyNIM03420080069WINNY_JANUARTI@YAHOO.COM
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorHardoko, HardokoNIDN9903005173UNSPECIFIED
Thesis advisorSiregar, Tagor M.NIDN0304016902UNSPECIFIED
Uncontrolled Keywords: antioxidant; Column Chromatography; edible bird’s nest; extraction; Thin Layer Chromatography
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Mrs Marselita Harapan
Date Deposited: 16 Oct 2018 08:06
Last Modified: 05 Aug 2020 03:37
URI: http://repository.uph.edu/id/eprint/1918

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