Sikap wirausaha, berpikir kritis dan penguasaan konsep merupakan hal-hal yang penting dalam proses pembelajaran di dalam kelas terutama dalam memperlengkapi siswa di era industri 4.0. Penelitian ini bertujuan untuk mengetahui adanya perbedaan sikap wirausaha, berpikir kritis, dan penguasaan konsep siswa yang menggunakan model project-based learning berbasis chemo-entrepreneurship dengan metode ceramah. Penelitian dilakukan pada siswa kelas 9 di Sekolah XYZ Bogor pada bulan Februari-Mei 2020, dengan subjek penelitian sebanyak 15 orang di masing-masing kelas baik eksperimen maupun kontrol. Penelitian ini merupakan penelitian kuantitatif menggunakan metode weak experiment dengan static-group pretest and postest design. Data diperoleh dengan rubrik dan tes kemudian dicari nilai rata-rata dan n-gain. Untuk melihat perbedaan antara kelas eksperimen dan kontrol digunakan uji Mann-Whitney. Hasil dari penelitian ini adalah terdapat perbedaan sikap wirausaha, keterampilan berpikir kritis dan penguasan kosep kimia siswa di kelas eksperimen dan kontrol. Uji sikap wirausaha diperoleh hasil asymp. sig (2-tailed) = 0,000, uji keterampilan berpikir kritis diperoleh hasil asymp. sig (2-tailed) = 0,000, dan uji nilai posttest penguasaan konsep diperoleh hasil asymp. sig (2-tailed) = 0,015 dan asymp. sig (2-tailed) = 0,011 untuk nilai n-gain, dengan hasil kurang dari 0,05. Maka terdapat perbedaan sikap wirausaha, keterampilan berpikir kritis dan penguasaan konsep antara kelas eksperimen dengan kontrol. / Entrepreneurial attitudes, critical thinking skills and mastery of concepts are important during student’s learning process in the classroom especially equipping them to perform well in Industry 4.0 era. The objectives of this research were to identify the differences of students’ entrepreneurial attitudes, critical thinking skills and mastery of chemistry concepts from the class using project-based learning model as experimental class and direct instruction method as control class. This research was conducted to grade 9 students in XYZ school in Bogor on February–May 2020. The number of participants in this research are 15 students for experimental and control class. The research is quantitative research using weak experiment method and static-group pretest and post-test design. The research data were collected through rubrics and test. The data analysis used descriptive statistics such as average and n-gain score and inferential statistic such as Mann-Whitney test. The result of this research is students’ entrepreneurial attitudes, critical thinking skills and mastery of chemistry concepts from experimental and control class are different. It can be seen through the asymp. sig (2-tailed) of entrepreneurial attitudes = 0,000, critical thinking skills = 0,000, and the post-test and n-gain scores from mastery of chemistry concepts = 0,015 and 0.011 which are below than 0,05. To sum up, there are differences of students’ entrepreneurial attitudes, critical thinking skills and mastery of concepts from the experimental and control class.