Karakteristik ekstrak kasar daun torbangun (Plectranthus amboinicus [Lour.] Spreng.) sebagai antibakteri dan stabilitasnya terhadap bakteri patogen = Characteristic of indian borage crude extract (plectranthus amboinicus [lour.] spreng.) as antibacterial and its stability towards pathogenic bacteria

Halim, Valencia Sunardi (2020) Karakteristik ekstrak kasar daun torbangun (Plectranthus amboinicus [Lour.] Spreng.) sebagai antibakteri dan stabilitasnya terhadap bakteri patogen = Characteristic of indian borage crude extract (plectranthus amboinicus [lour.] spreng.) as antibacterial and its stability towards pathogenic bacteria. Bachelor thesis, Universitas Pelita Harapan.

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Abstract

Pada studi sebelumnya daun torbangun diketahui mengandung komponen yang bersifat sebagai antibakteri seperti fenolik, flavonoid, tanin, saponin, dan steroid. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas penghambatan dari ekstrak etanol, etil asetat dan heksana daun torbagun terhadap bakteri patogen seperti S. Thypi, E.coli, P. aeruginosa, S. aureus and L. monocytogenes menggunakan metode difusi sumur. Konsentrasi ekstrak yang digunakan adalah 5%, 10%, 15%, dan 20%. Hasil menunjukkan bahwa ekstrak daun torbangun dapat mengambat pertumbuhan bakteri P. aeruginosa, S. aureus, L. monocytogenes, namun tidak dapat menghambat E. Coli dan S. Thypi. Ekstrak etanol, etil asetat dan heksana daun torbangun dengan konsentrasi terpilih kemudian dilanjutkan dengan uji stabilitas terhadap garam, gula, pH, dan pemanasan. Selain itu, juga dilakukan uji toksisitas pada ekstrak terpilih. Ekstrak daun torbangun stabil pada garam 5%-10%, gula 10-%50%, pH 5-6. Pada suhu pemanasan 100°C selama 15 menit, ekstrak daun torbangun masih menunjukkan aktivitas antibakteri. Hasil dari uji toksisitas menyatakan bahwa ekstrak etanol torbangun tidak toksik, namun ekstrak etil asetat dan heksana dari daun torbangun dikategorikan toksik./ Previous study had shown that Indian Borage leaves have antimicrobial compound such as phenolic, flavonoid, tannin, saponin and steroid. The purpose of this research was to know the inhibition activity of ethanol, ethyl acetate, and hexane extract of indian borage leaves towards pathogenic bacteria such as S. Thypi, E.coli, P. aeruginosa, S. aureus and L. monocytogenes through well diffusion method. Extract concentration used are 5%, 10%, 15% and 20%. The results showed that the Indian Borage extract was able to inhibit the growth of P. aeruginosa, S. aureus, and L. monocytogenes, however could not inhibit the growth of E.coli and S. Thypi. The Ethanol, ethyl acetate, and hexane extract of indian borage leaves with chosen concentration 5% was proceeded to the stability test toward salt, sugar, pH and heat. In addition, toxicity test was also performed to the selected indian borage extract. The indian borage leaves extract was stable towards concentration of 1%-5% salt, 10%-50% sugar, and pH 5-6. At 100°C 15 minutes of heating, indian borage extract still shown the antibacterial activity. The results of toxicity test stated that the ethanol indian borage leaves extract is non toxic, however the ethyl acetate and hexane extract of indian borage leaves extract are categorized toxic.

Item Type: Thesis (Bachelor)
Creators:
CreatorsNIMEmail
Halim, Valencia SunardiNIM00000011650valenciash21@gmail.com
Contributors:
ContributionContributorsNIDN/NIDKEmail
Thesis advisorParhusip, Adolf Jan NexsonNIDN0107016701UNSPECIFIED
Uncontrolled Keywords: antibakteri; daun torbangun; Plectranthus amboinicus (Lour.) Spreng; bakteri patogen; difusi sumur;
Subjects: T Technology > TP Chemical technology > TP368-456 Food processing and manufacture
Divisions: University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology
Depositing User: Users 2342 not found.
Date Deposited: 20 Feb 2020 06:51
Last Modified: 14 Oct 2020 05:01
URI: http://repository.uph.edu/id/eprint/7641

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