Aretha, Devanna (2025) Potensi probiotik bifidobacterium bifidum dan lactobacillus acidophilus pada berbagai rasio untuk menurunkan kadar logam besi, aluminium, dan zink = Potential of bifidobacterium bifidum and lactobacillus acidophilus probiotics at various ratios for reducing the level of iron, aluminum, and zinc metals. Bachelor thesis, Universitas Pelita Harapan.
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Abstract
Pencemaran dan akumulasi logam berat pada pangan adalah salah satu masalah lingkungan yang berdampak pada pangan. Pemanfaatan probiotik untuk mengurangi kandungan logam berat pada lingkungan dan pada bidang pangan adalah salah satu solusi biologis yang aman dan telah diterapkan. Tujuan dari penelitian ini adalah untuk menentukan rasio probiotik Lactobacillus acidophilus dan Bifidobacterium bifidum (1:0, 0:1, 1:1, 1:2, 2:1) dan jenis logam (Fe, Al, Zn) yang paling optimal untuk mengurangi logam. Penelitian pendahuluan dilakukan untuk melihat karakteristik dari probiotik, kemampuan resistensi, kurva pertumbuhan, dan jumlah bakteri starter terhadap rasio probiotik. Penelitian utama terdiri dari pengujian jumlah bakteri, berat kering sel, kemampuan biosorpsi, dan kemampuan akumulasi terhadap rasio probiotik dan jenis logam. Hasil penelitian pendahuluan uji resistensi menunjukan MIC untuk logam Fe, Al, dan Zn adalah 616 ppm, >840 ppm, dan >1680 ppm. Jumlah bakteri starter tertinggi adalah pada rasio probiotik 2:1 (9,21 ± 0,08 log CFU/ mL). Hasil berat kering sel tertinggi ada pada rasio probiotik 0:1 dengan logam Fe (0,2464 ± 0,2044 g). Jumlah bakteri probiotik setelah dikontak dengan logam tertinggi adalah pada rasio probiotik 0:1 logam Zn (9,77 ± 0,15 log CFU/mL). Hasil biosorpsi tertinggi adalah rasio probiotik 1:0, 0:1, 1:2, dan 2:1 untuk logam Al (99,99 ± 0,00%). Hasil akumulasi tertinggi adalah pada rasio probiotik 1:2 untuk logam Fe (623,48 ± 149,82 mg/g). Perlakuan terbaik dari penelitian ini adalah rasio probiotik 1:2 dan untuk menurunkan kadar logam Al.
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Heavy metal contamination and accumulation is one of the environmental problems that impact the food industry. Probiotics is one of the health-safe biological solutions that has been implemented that can be used to reduce heavy metal content in the environment and food. The purpose of this study was to determine the probiotics ratio Lactobacillus acidophilus and Bifidobacterium bifidum (1:0, 0:1, 1:1, 1:2, 2:1) and metal types (Fe, Al, Zn) that have the biggest potential to reduce metals. Preliminary research was conducted to see the characteristics of probiotics, resistance ability, growth curve, and the number of starter bacteria to the probiotic ratio. The main research consisted of finding the number of bacteria, dry cell weight, biosorption, and accumulation to the ratio of probiotics and types of metals. The results of the preliminary research resistance test showed that the MIC for Fe, Al, and Zn metals are 616 ppm, >840 ppm, and >1680 ppm. The highest number of starter bacteria is for the probiotic ratio of 2:1 (9.21 ± 0.08 log CFU/mL). The highest dry cell weight result is at probiotic ratio of 0:1 with Fe metal (0.2464 ± 0.2044 g). The highest number of probiotic bacteria after contacting with metal is at probiotic ratio of 0:1 Zn metal (9.77 ± 0.15 log CFU/mL). The highest biosorption results are for probiotic ratios of 1:0, 0:1, 1:2, and 2:1 for Al metal (99.99 ± 0.00%). The highest accumulation result is at ratio of 1:2 for Fe metal (623.48 ± 149.82 mg/g). The best treatment in this research is at probiotics ratio of 1:2 for reducing the level of Al metal.
Item Type: | Thesis (Bachelor) |
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Creators: | Creators NIM Email ORCID Aretha, Devanna NIM01034210017 devanna.arth@gmail.com UNSPECIFIED |
Contributors: | Contribution Contributors NIDN/NIDK Email Thesis advisor Parhusip, Adolf Jan Nexson NIDN0107016701 adolf.parhusip@uph.edu |
Uncontrolled Keywords: | probiotik; logam; rasio probiotik; biosorpsi; akumulasi; probiotics; metals; probiotics ratio; biosorption; accumulation. |
Subjects: | T Technology > TP Chemical technology > TP368-456 Food processing and manufacture |
Divisions: | University Subject > Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology Current > Faculty/School - UPH Karawaci > Faculty of Science and Technology > Food Technology |
Depositing User: | Stefanus Tanjung |
Date Deposited: | 11 Apr 2025 08:35 |
Last Modified: | 11 Apr 2025 08:35 |
URI: | http://repository.uph.edu/id/eprint/68073 |